摘要
目的:探讨TGF-β1在腺样囊性癌的增殖、迁移和侵袭、浸润过程中的作用和相关机制。方法:以腺样囊性癌ACC-2细胞株为研究对象,采用TGF-β1刺激ACC-2细胞,MTT法检测ACC-2细胞的增殖能力,Transwell实验检测细胞迁移、侵袭能力,Western蛋白印迹检测ACC-2细胞MAPK(P38、JNK、ERK)的活化及MMP-2表达的变化,实时定量PCR检测ACC-2细胞中MMP-2的mRNA表达变化。采用SPSS17.0软件包对数据进行统计学分析。结果:TGF-β1刺激后,ACC-2细胞增殖能力无显著变化(P>0.05),但迁移、侵袭能力增强(均为P<0.01);同时,ACC-2细胞p-ERK1/2、p-P38和MMP-2蛋白表达升高(均为P<0.05),MMP-2 mRNA表达亦升高(P<0.01),但p-JNK1/2蛋白无显著变化(P>0.05)。结论:TGF-β1可增强人唾液腺腺样囊性癌ACC-2细胞的迁移和侵袭能力,活化p-ERK1/2和p-P38,上调MMP-2的表达。TGF-β1/MAPK/MMP-2通路可能参与人唾液腺腺样囊性癌ACC-2细胞侵袭能力的调节。
PURPOSE： To investigate the role of TGF-β1/ MAPK/ MMP-2 signaling pathways and the relation with its biological behavior in salivary adenoid cystic carcinoma.METHODS： ACC-2 cell line was used and resuscitated by TGF-β1 in this study.Cell proliferation ability was observed by MTT assay;cell migration and invasion potential were examined by Transwell;the expression of MMP-2 was tested by Western blotting and real-time PCR;the activity of MAPK signaling pathway was measured by Western blotting in ACC-2 cell lines.All data were analyzed by SPSS17.0 software package.RESULTS： TGF-β1 did not enhance cell proliferation ability of ACC-2 cells（P0.05）,but enhanced cell migration and invasion potential significantly（P0.01）.The protein expression of MMP-2,p-ERK1/2 and p-P38 and the mRNA expression of MMP-2 increased（P0.05） while p-JNK1/2 was not increased in stimulated ACC-2 cells by TGF-β1（P0.05）.CONCLUSION： TGF-β1 can enhance migration and invasion potential,and activate ERK1/2 and P38,and up-regulate MMP-2 expression in ACC-2 cells.The invasion potential of salivary adenoid cystic carcinoma might be regulated by TGF-β1/ MAPK/ MMP-2 signaling pathway.
出处
《中国口腔颌面外科杂志》
CAS
2012年第2期101-106,共6页
China Journal of Oral and Maxillofacial Surgery
基金
广东省自然科学基金(9151008901000041)
广东省科技计划国际合作项目(2010B050700005)~~
