摘要
为建立以高效液相色谱法(HPLC)测定罗库溴铵中溴丙烯的含量的方法,实验色谱条件为,色谱柱为C18(Kromasil 250 mm×4.6 mm,5μm),流动相为0.05 mol.L-1四甲基氢氧化铵溶液(用磷酸调节pH值至5.0)—乙腈(4∶6),流速为1.0 mL.min-1,检测波长为210 nm,进样量为5μL.结果表明,以溴丙烯浓度(C)对峰面积(A)进行回归分析,回归方程为,A=34337.41C-2682.68,r=0.9999.在检测浓度为,0.528μg/mL～105.6μg/mL范围内呈良好线性关系,平均回收率为97.6%～100.2%,RSD=0.9%(n=9),精密度试验RSD=0.9%.实验证实,采用HPLC法测定罗库溴铵中溴丙烯的含量,方法灵敏度高,专属性强,结果准确可靠.
To establish HPLC method for the detennination of the content of allyl bromide in rocuronium bromide,Cls（Kromasil 250 mmx4.6 mm,5 tan） was used as chromatographic column,0.05 mol-L^-1 of whose ratio of tetramethylammonium hydroxide （pH adjusted to 5.0 with phosphoric acid） to acetonitrile is 4： 6 was used as mobile phase, with flow velocity of 1.0 mL· min^-1, determination wavelength of 210 nm and sample size of 5 μL.The regression equation of A = 34337.41C- 2682.68 based on the allybromide concentration （C） and peak area （A） is obtained,r = 0. 9999, showing good linearity within the detection concentration of 0.528 μg/mL -105.6 μg/mL.The average recovery rate is 97.6% - 100.2% and preci- sion test RSD is 0.9% （n = 9）. It is verified by the experiment that this method is exclusive, sensitive and reliable for content determination of allybromid in Rocuronium Bromide.
出处
《成都大学学报（自然科学版）》
2012年第1期14-16,20,共4页
Journal of Chengdu University（Natural Science Edition）
