摘要
目的建立高效液相色谱(HPLC)法测定罗库溴铵原料的含量。方法采用氨基键合硅胶为填充剂,以0.04mol/L四甲基氢氧化铵溶液(用磷酸调节pH值至7.4)-乙腈(10∶90)为流动相,柱温35℃,检测波长207nm。结果线性范围为0.1251～2.0065mg/ml(r2=0.9999);平均回收率为99.9%(RSD=0.1%)。结论采用HPLC法测定罗库溴铵原料的含量,方法灵敏度高,专属性强,结果准确可靠。
Objective To establish a HPLC method to determinate the content of rocuronium bromide.Methods NH2-bond-ing silica gel was used as the stationary phase.The mobile phase consisted of 0.04 mol/L tetramethyl ammonium hydroxide（pH adjus-ted to 7.4 by phosphoric acid）and acetonitrile（10：90）.The column temperature was 35℃.The detection wavelength was 207 nm.Results The relation between concentration and peak area was linear in the range of 0.125 1-2.006 5 mg/ml with r^2 of 0.999 9.The average recovery rate was 99.9% with RSD of 0.1%.Conclusion This method is exclusive,sensitive and is reliable for the determi-nation of rocuronium bromide.
出处
《国际药学研究杂志》
CAS
2010年第2期127-129,共3页
Journal of International Pharmaceutical Research
