摘要
利用脂肪酶BSL2作为催化剂,在有机介质中催化合成丁酸乙酯.考察了反应温度、底物浓度、底物摩尔比、反应介质和初始水活度等反应条件对酶促合成丁酸乙酯的影响.试验结果表明:以正己烷为溶剂,初始水活度为0.33,加酶量为100 mg,丁酸浓度为40 mM,底物摩尔比(丁酸/乙醇)为1∶1.5,在40℃条件下振荡反应30 h,合成丁酸乙酯的的转化率可达到94.8%.
Bacillus subtilis lipase（BSL2） was used to catalyze the synthesis of ethyl butyrate in organic sol- vents. The effects of reaction conditions such as reaction temperature, concentration of substrate, molar ratio of substrate, reaction medium, initial water activity and others on the synthesis of ethyl butyrate catalyzed by lipase were investigated to obtain the optimum reaction conditions. The findings show that percent conver- sion of synthesis of ethyl butyrate reaches 94.8% under the optimum conditions of using n-hexane as solu- tion,0.33 of initial water activity,with 100 mg lipase and 40 mM concentration of butyric acid, 1 ： 1.5 of molar ratio of substrate, at the temperature of 40℃ after 30 h oscillatory reaction.
出处
《成都大学学报（自然科学版）》
2012年第1期4-7,共4页
Journal of Chengdu University（Natural Science Edition）
基金
国家自然科学基金(31070708
20803028
30870539)
吉林大学科学前沿与交叉学科创新项目(200903099)资助项目
关键词
丁酸乙酯
脂肪酶
有机介质
酯合成反应速度
ethyl butyrate
lipase
organic medium
percent conversion of synthesis of ethyl butyrate
