摘要
目的:该研究探讨维甲酸对Bel7402人肝癌细胞的分化诱导作用,为肝癌的分化治疗提供资料。方法:用维甲酸处理Bel7402人肝癌细胞,观察细胞增殖、软琼脂集落形成和各项生化指标的变化。结果:10μmol/L维甲酸显著抑制肝癌细胞增殖,并使肝癌细胞软琼脂集落形成率明显减少,使代表肝细胞分化的酶鸟氨酸氨基甲酰转移酶、酪氨酸α酮戊二酸转氨酶和碱性磷酸酶比活力明显升高,甲胎蛋白分泌量、γ谷氨酰转肽酶和醛缩酶比活力明显下降。结论:证明维甲酸是Bel7402人肝癌细胞有效的分化诱导剂。
To investigate the induction effects of retinoids on the differentiation of cultured Bel-7402 Human Liver cancer cells.Methods:We observed the cell proliferation, colony forming test on soft agar, and the changes of each biochemical marker, after having treated Bel-7402 Human Liver cancer cells with retinoids.Results:10 μmol/L retinoids could inhibit the Bel-7402 proliferation and decrease the colony forming of liver cancer cells on soft agar significantly. So some biochemical markers indicating the proliferation status of liver cancer cells changed significantly, including the significant activity increase of ornithine carbamyl transferase (OCT), tyrosine-α-ketoglutaric transaminase (TAT) and alkaline phosphatase (AKP), the significant decrease α-fetoprotein (AFP) secretion, γ-glutamyltranspetidase and aldolase activity.Conclusions:The results suggest that retinoids is an effective differentiation revulsiva of Bel-7402 human liver cancer cells.
出处
《中国现代医学杂志》
CAS
CSCD
2000年第2期15-16,共2页
China Journal of Modern Medicine
关键词
肝癌
细胞分化
维甲酸
酶学
甲胎蛋白
Liver Cancer
Cell Differentiation
Retinoids
Enzymology
α-Fetoprotein (AFP)
