摘要
目的:建立一种简便、灵敏的测定人体血浆中吲哒帕胺浓度的高效液相色谱-串联质谱(HPLC-MS/MS)方法。方法:以瑞格列奈钙为内标,采用乙腈:0.1%甲酸水溶液=90∶10为流动相,以Agilent-ZORBAX-C18柱(2.1mm×150mm,5.0m)色谱柱为分析柱,通过电喷雾离子源(ESI),以正离子多反应监测(MRM)方式进行进样检测。检测离子为[M+H]+m/z 364.1m/z 188.8(吲哒帕胺)和[M+H]+m/z 451.1m/z 134.8(瑞格列奈钙,内标)。结果:吲哒帕胺线性范围为0.5～50ng/mL,定量下限为0.5ng/mL,线性关系良好;高、中、低三个浓度的日内、日间的RSD均<15%,平均回收率为94.60%～110.33%。结论:本方法专属性强,操作简便,灵敏度高,适用于吲哒帕胺制剂的临床药动学研究。
AIM： To establish a simple, sen- sitive method of high performance liquid chroma- tography tandem mass spectrometry （HPLC- MS/MS） for the determination of Indapamide in human plasma. METHODS： Repaglinide calcium was used as internal standard. Indapamide was separated on an Agilent-ZORBAX-Qs column （2.1 mm× 150 mm,5.0 μm）. The mobile phase consisted of acetonitrile-- 0.1 G formic acid （90 ： 10）. Electrospray ionization（ES1） was applied and operated in the positive multiple reaction monitoring（MRM） mode,used the transitions of [M＋ H]＋m/z 388.4 m/z 247.4 and [M＋H] ＋m/z 384.2 m/z 231.3 to quantify Indapamide and the internal standard respectively. RESULTS： The linear calibration curve was ob- tained over the concentration range of 0.5-- 50 ng/mL. The limit of quantification was 0.5 ng/mL. The inter- and intra-day precision （RSD） were less than 15%. The average recovery was between 94.60% and 110.33%. CONCLUSION. The method had been proved to be specific, convenient, sensitive and suitable for the clinical investigation of Indapamide pharmacoki- netics.
出处
《中国临床药理学与治疗学》
CAS
CSCD
2012年第2期206-210,共5页
Chinese Journal of Clinical Pharmacology and Therapeutics
