摘要
在碱性条件下用二氯甲烷提取,以甲磺酸多沙唑嗪为内标,采用XDBC18反相柱(5μ),质谱检测器采用AP-ESI,正离子模式,采集方式为SIM,流动相为乙腈-水-醋酸-醋酸铵(50∶50∶0.1∶0.2,V/V),用液相色谱-质谱联用法(LC-MS)测定人血浆中吲哒帕胺浓度。吲哒帕胺在1—150ng/mL范围内线性关系良好,提取回收率大于80%,精密度(RSD)均小于10%,检出限为1.0ng/mL。该法方便、准确、灵敏,适用于吲哒帕胺缓释制剂的药代动力学研究,也可以用于临床测定吲哒帕胺血浆药物浓度。
The amount of indapamide in human plasma was determined by LC MS with doxazosin mesylate as internal standard in reversed-phase Inertsil C18 column（5μ）and mobile phase of acetonitrile ： water ＂ acetic acid ＂ ammonium acetate（50 ： 50 ： 0. 1 ： 0. 2,V/V） at flow rate of 1.0mL/min. The plasma was extracted by dichloromethane in basic condition. Detection is performed with an electrospray ionization mass spectrometer in positive ion and selected-ion monitoring mode. Indapamide was separated completely under the optimal conditions in the linear range of 1. 0- 150ng/mL with the RSD of less than 10%, the average recovery of more than 80% and detection limit of 1. 0ng/mL. The method is convenient, rapid, accurate and suitable for determination of Indapamide in human plasma and pharmacokinetic studies.
出处
《光谱实验室》
CAS
CSCD
2006年第2期324-327,共4页
Chinese Journal of Spectroscopy Laboratory
