摘要
目的观察宁夏地区栽培甘草(Glycyrrhiza uralensis Fisch.)提取物甘草黄酮(licorice flavonoids,LF)对2型糖尿病(T2DM)模型大鼠的降糖作用及抗氧化作用的研究。方法采用高脂高糖饮食喂养后(12周)注射链脲佐菌素(streptozotocin,STZ,35mg.kg-1,ip)制备大鼠2型糖尿病模型,实施腹腔注射300mg.kg-1和100mg.kg-1剂量的LF干预40d后,测定空腹血糖、血浆糖化血红蛋白(HbA1C)、尿糖值、24h尿蛋白值和酮体值,以及血清超氧化物歧化酶(SOD)、丙二醛(MDA)、谷胱甘肽过氧化物酶(GSH-PX)和总抗氧化能力(T-AOC)。结果 LF降低空腹血糖、HbA1C水平、尿糖值,升高血清SOD和GSH-PX水平的同时,降低血清MDA含量。LF对血浆T-AOC、24h尿蛋白值和酮体值,改善不明显。结论宁夏地区栽培甘草中甘草黄酮具有改善实验性2型糖尿病大鼠糖代谢的作用,其抗氧化活性可能与其作用有关。
Objective To investigate the hypoglycemic effect of licorice flavonoids （LF） from cultivated lico- rice （Glycyrrhiza uralensis Fisch. ） on type II Diabetes Mellitus and its related mechanism in rats. Methods Rat model of type II diabetes mellitus （DM） was established with long- term high fat and sugar diet （12w） and intraperitoneal injection of low - dose streptozotocin （STZ, 35mg·kg-l ）. After implementation of the 300mg·kg-1 and 100mg·kg-1 doses of LF intragastrically adminstration over 40 days, the fasting blood glu- cose value, plasma glycated hemoglobin （HbA1 C） , urine sugar value, 24hr urine protein value and the ke- tones values, and serum superoxide dismutase （SOD）, malondialdehyde （MDA）, glutathione peroxidase （ GSH - PX） and total antioxidant capacity （ T - AOC ） were analyzed at the end of the test. Results LF could lower the fasting blood glucose value, lower HbA1C levels, and reduced urine sugar value ; LF could in- crease the serum levels of SOD and GSH - PX, while reducing serum MDA level. LF did no significantly im- prove the plasma T -AOC, 24hr urine protein value and the ketones value. Conclusion Ningxia cultivated licorice flavonoids can improve experimental II diabetes glucose metabolism and the antioxidant activity may be related to the effect.
出处
《宁夏医科大学学报》
2012年第2期110-114,共5页
Journal of Ningxia Medical University
基金
国家中医药国际科技合作计划项目(2006DFA33680)
宁夏医科大学重点孵育项目(XZ201002)
关键词
甘草
黄酮
糖尿病
脲佐菌素
Glycyrrhiza uralensis Fisch.
flavonoids
diabetes mellitus
streptozotocin
