摘要
以油茶优良无性系的腋芽为外植体,分别采用附加不同种类激素的MS培养基对其进行组织培养试验,结果表明:不定芽的最佳继代增殖培养基为MS+6-BA0.8 mg.L-1+NAA0.1 mg.L-1,诱导生根最适培养基为1/2MS+IBA0.5 mg.L-1+NAA0.2 mg.L-1,生根率达87.5%。
Axillary buds of Camellia oleifera,which was used as explants,were added MS culture medium with different kinds of hormone.Result shows that the optimal medium for shoot regeneration from auxiliary buds is MS ＋6-BA 0.8 mg·L-1＋NAA 0.1 mg·L-1;the optimal medium for root inducement was suggested as 1/2MS ＋IBA 0.5 mg·L-1＋ NAA 0.2 mg·L-1.Rooting rate are 87.5%.
出处
《防护林科技》
2012年第2期24-26,共3页
Protection Forest Science and Technology
关键词
油茶
无性系
组织培养
Camellia oleifera
clones
tissue culture
