摘要
对虾夷马粪海胆“黑嘴病”进行了初步的研究。经分离、纯化后鉴定黑嘴病的病原菌为坚强芽孢杆菌 (BacillusfirmusBredemannetWarner)。同时报道了该菌为革兰氏阳性杆菌 ,大小为(0.6～0.7)μm×(0.8～1.0)μm。可在1%～7%NaCl中生长 ,最适盐度2～3 ;最适pH为7～8;生长温度为8～26℃ ,最适生长温度为15℃。该菌对磺胺甲基异恶唑、复方新诺明和头孢噻肟等抗生素敏感 。
This paper reported the study of black mouth disease of Strongylocentroatus intermedius.The pathogen were isolated and purified from disease Sea Urchin of Dalian Pacific Seafood Company. They were gram positive rod bacteria, size (0.6 0.7)μm×(0.8 1.0) μm.There are gemma but no nflated sporosa in the cell. Catalase was positive and oxidase negative. There are no PHB granules in their cells. They grew in 1% 7%NaCl and pH 6 13 at temperature 8 26 ℃ but not grow in pH 5.7. They do not produce acetyl methyl carbinol. They reduced nitrate to nitrite, fermented amylum, glucose, xylose, arabinose and mannite, but glucose and xylose produced acid. They cannot grow in anaerobic medium. This bacteria was identified as Bacillus firmus Bredemann et Warner. The sensitivity of this bacteria to 44 kinds of antibiotics was also studied. They were sensitive to Sulfamethoxazole (the diameter of ring for bacteria inhibition (DRBI)was 31.0 mm), Cotrimoxazol (DRBI was 28.0 mm ), cefotaxime(DRBI was 26.0 mm),Ceftriaxone (DRBI was 23.0 mm),Spectinomycin (DRBI was 23.0 mm), Ceftazidime (DRBI was 19.0 mm); and resistant to Chloramphenical,Gentamicin,Ampicillin, Streptomycin, Neomycin, Midecamycin, Cefradine, Cefalexin, Amikacin, Norvancomycin, etc. The results suggested that some kinds of antibiotics can be used to control this disease.
出处
《海洋科学》
CAS
CSCD
北大核心
2000年第3期41-43,共3页
Marine Sciences
基金
农业部重点资助项目!95A960402号
关键词
虾夷马粪海胆
黑嘴病
坚强芽孢杆菌
抗生素
海胆
Strongylocentrotus intermedius, Black mouth disease, Bacillus firmus, Antibiotics
