摘要
于1993年6月在大连太平洋海珍品养殖公司的12个采集点分离到河流弧菌Ⅱ的噬菌体，依据过量噬菌体可以全部裂解其宿主菌河流弧菌Ⅱ的特性，用噬菌体对皱纹盘鲍脓疱病进行了生物防治研究。结果表明，使用一定浓度的噬菌体可以有效地治疗或推迟脓疱病引起的鲍死亡，可将鲍的成活率提高50％以上。探讨了在噬菌体生物防治中遇到的难题及解决办法、噬菌体分离规律等问题。
The isolation, purification and propagation of bacteriophage of Vibrio fluvialis-Ⅱ were studied. The phage was isolated from 12 different water smples, using the normal isolation procedure. The Vibrio fluvialis-Ⅱ can grow well on STA (seawater, tryptone and agar) medium,which was used as a host and test culture.The plaqus were obtalned by the agar bilayer method. Concentrated phage suspensions were obtalned from plates by washing them with 2% NaCl solution (e.g. add 4ml 2% NaCl solution into each plate), they were then put into refrigerator at 4t overnight. The phage suspensions from the plate were addd into a 5ml tube. The host bacteria were removed by centrifugation at 8 000r/min and filter, and were purified and propagated by picking the single plaque repeatedly.The bacteria merio j7uviallerlI can be all split using the phage with a high concentration. The effect of basteriophage control pustule disease of abalone by muscular injection and infection on the wound in abalone foot can raise the survival rate of the abalone by up to 50%.Electron microscopic exdrination of the material takn from the plaques of the phage show that they contained simulboeously tWo forms of phage panicles, with laree and small heads, while their tails were morphologically identical. Numerous successive passages of the material taken from single plaqu did not make it possible to septal small and large phage particles.
出处
《海洋与湖沼》
CAS
CSCD
北大核心
1999年第4期374-380,共7页
Oceanologia Et Limnologia Sinica
基金
大连市科学技术委员会资助![1995]42
关键词
皱纹盘鲍
脓疱病
噬菌体
生物防治
Haliotis discus hannai
Pustule disease
Bacteriophape
Biological control
