摘要
目的研究二氢杨梅素(DMY)对Cu2+诱导的β淀粉样蛋白片段(Aβ1-40)聚集和细胞毒性的影响。方法采用硫磺素T荧光标记研究DMY对Cu2+诱导Aβ1-40聚集的影响,MTT法检测SH-SY5Y细胞活力的变化。结果 DMY能明显抑制Cu2+诱导的Aβ1-40聚集,对已形成的Cu2+-Aβ1-40聚集物有解聚作用,且能有效防止Cu2+和Aβ1-40共同诱导的SH-SY5Y细胞活力的下降。结论 DMY对阿尔茨海默病具有潜在的防治作用。
Objective To investigate the effects of dihydromyricet（DMY） on the aggregation of β-amyloid （Aβ1-40） induced by Cu2＋ and the neurotoxicity of Cu2＋-Aβ1-40 aggregates. Methods The aggregation of Aβ1- 40 induced by Cu2＋ was detected by thioflavin T fluorescence spectroscopy, and the change of SH SY5Y cell viability was evaluated by MTT assay. Results DMY significantly inhibited Cu^2＋-induced aggregation of Aβ1-40 and depolymerized the preformed Cu^2＋-Aβ1-40 aggregates. Moreover,DMY could effectively prevent the decreasement of cell viability induced by Cu2＋ and Aβ1-40. Conclusion DMY may be taken as a neuroprotective agent for the treatment of Alzheimer＇s disease.
出处
《江苏医药》
CAS
CSCD
北大核心
2012年第6期641-643,共3页
Jiangsu Medical Journal
基金
江苏省高技术研究计划(医药部分)重点项目(BG2007607)
南通大学校级自然科学基金(10ZY017)
