摘要
研究藻红蛋白调控细胞内线粒体膜电位变化诱导人乳腺癌MCF-7细胞凋亡的研究.MTT法检测藻红蛋白对MCF-7细胞的细胞毒作用;倒置显微镜观察藻红蛋白对细胞形态学的影响;Ho-echst33258单染,荧光显微镜下观察藻红蛋白作用MCF-7细胞的形态学变化;PI单染,流式细胞术测定藻红蛋白诱导MCF-7细胞凋亡的凋亡率;TMRE单染,激光共聚焦显微镜检测MCF-7细胞中线粒体膜电位的变化.藻红蛋白对MCF-7细胞的IC50为81.60μg/L;形态学观察在作用48 h后,随给药质量浓度增加细胞生长密度逐渐变疏,细胞变小,细胞皱缩,高质量浓度组大部分细胞破碎,贴壁减少.Hoechst33258荧光染色结果显示,藻红蛋白与MCF-7细胞共培养后,随着剂量增加细胞呈明显的固缩状,细胞核出现碎片和凋亡小体等细胞凋亡现象;藻红蛋白作用48 h后的细胞出现凋亡的亚二倍体峰,DNA复制下降;凋亡率分别为25.18%、55.45%、83.94%.不同质量浓度藻红蛋白对MCF-7作用48 h后,线粒体膜电位荧光强度分别为24.40±0.21、20.45±0.24和19.77±0.27.藻红蛋白通过调节细胞内线粒体膜电位变化诱导MCF-7细胞凋亡.
To investigate the effect of phycoerythrin induces MCF - 7 apoptosis by controlling variation of △ψm. The inhibition of phycoerythrin on MCF -7 cells detected by MTT assay. Hoechst33258 staining was observed under fluorescence microscope role of phycoerythrin glycoprotein MCF- 7 cell morphology. Apoptosis rate of MCF -7 by phycoerythrin （FCM）. Effect of phycoerythrin on variation of △ψm in MCF -7 cells observed by CLSM. The phyco- erythrin can restrain the growth of tumor cell MCF - 7. The IC50 was 81.60μg/L for the method of MTT. The morphology of cell was observed by invert microscope in cell collapsed, break to pieces. Hoechst33258 staining showed obvious appearance of apoptosis： nuclear condensation and fragmentation, chromatin condensation, and apoptotic body formation. 40, 80, 160 μg/L of phycoerythrin on MCF - 7 the apoptosis rate of MCF - 7 by phycoerythrin was 25.18%, 55.45%, 83.94%. Result of phycoerythrin on variation of △ψm in MCF -7 cells observed by CLSM. 24.40± 0.21; 20.45 ± 0.24, 19.77 -0.27 （FI）. Phycoerythrin induces MCF- 7 apoptosis by controlling variation of △ψm
出处
《哈尔滨商业大学学报（自然科学版）》
CAS
2012年第1期8-13,共6页
Journal of Harbin University of Commerce：Natural Sciences Edition
