摘要
以海洋经济海藻——龙须菜(Gracilaria lemaneiformis)为材料,机械破碎与超声波相结合破碎这种含胶量非常多的细胞,蔗糖密度梯度超速离心纯化其类囊体膜,去垢剂Triton X-100增溶纯化的类囊体膜,再用蔗糖密度梯度超速离心方法分离其叶绿素-蛋白质复合物。P700差示光谱鉴定分离的光系统Ⅰ(PSⅠ)颗粒,并且检测到了具有DCIP光还原活性的光系统Ⅱ(PSⅡ)颗粒。结果表明,尽管海藻类囊体膜增溶困难,但只要条件合适,可以得到具有活性的光系统颗粒。
Gracilaria lemaneiformis, an economical seaweed containing a lot of polysaccharide inthe cell, was homogenized by means of the combination of the mechanical grinding and ultrasonicdisruption, and then the thylakoid membrane was purified by the sucrose gradient centrifugation.The intrinsic chlorophyll-protein complexes with different pigment composition were isolated by thecentrifugation after the purified thylakoid membrane had been fragmented with the non-ionic detergent,Triton X-100. Differential spectrophotometry of these isolated chlorophyll-protein complexes showedthat the photosystemⅠwas obtained, and the photosystemⅡ was detected to have the activity ofphoto-reduction for DCIP. All these results indicated that the photosystem particles with activitiescan be obtained although the marine algal membranes are extremely resistant to attack by detergents.
出处
《植物学通报》
CSCD
北大核心
2004年第4期449-454,共6页
Chinese Bulletin of Botany
基金
国家自然科学基金重大项目(39890390)资助。
