摘要
目的:建立花椒与青椒的HPLC指纹图谱,并比较二者的差异。方法:样品用50%甲醇25 mL超声提取15 min,采用HPLC法建立指纹图谱,色谱条件为Hypersil BDS C18(250 mm×4.6 mm5,μm)色谱柱,甲醇-水梯度洗脱,检测波长268 nm,柱温35℃,流量1.0 mL/min,分析时间130 min。分别采用"中药色谱指纹图谱相似度评价系统(2004A版)"和SPSS 17.0对谱图进行相似度分析和聚类分析。结果:花椒和青椒HPLC指纹图谱的相似度分别为0.909～0.992、0.930～0.999,二者分别有27和24个共有峰,从标准指纹图谱和聚类分析结果看,二者存在明显差异。结论:本法简单、准确、快捷,可明显区别花椒与青椒,建议将HPLC指纹图谱纳入花椒药材的质量控制指标。
Objective ： To establish the HPLC fingerprint of Zanthoxylum bungeanum and Zanthoxylum schinifolium for finding the difference. Methods： Samples were extracted with 50% methanol 25 mL by ultrasonic wave and then separated on Hypersil BDS Cls （250 mm x 4. 6 mm,5 μm）column. Gradient elution was carried out with a mobile phase of methanol-water. The detection wavelength was 268 nm, the column temperature was set at 35℃, the flow rate was 1.0 mL/min and the analytic time was 130 min. The software ＂Similarity Evaluation System for Chromatographic Fingerprint of TCMs＂ （ Version 2004A） was employed to generate the mean chromat- ogram and carry out the similarity analysis of the samples. SPSS 17.0 was employed to carry out the cluster analysis. Results： Similari- ty of Z. bungeanum was 0. 909 - 0. 992 and that of Z. schinifolium was 0. 930 - 0. 999. There were 27 common peaks in HPLC Finger- prints of Z. bungeanum and 24 in that of Z. schinifolium. Their HPLC standard fingerprints were obvious difference. They belonged to different categories in cluster analysis. Conclusion： The method is simple, accurate and rapid. It could obviously distinguish Z. bun- geanum from Z. schinifolium. So it suggests that HPLC fingerprints should be one of the quality control indexes of Zanthoxyli Pericarpium.
出处
《中药材》
CAS
CSCD
北大核心
2012年第1期39-42,共4页
Journal of Chinese Medicinal Materials
关键词
花椒
青椒
指纹图谱
HPLC
Zanthoxylum bungeanum Maxim.
Zanthoxylum schinifolium Sieb. et Zucc.
Fingerprint
HPLC
