摘要
目的探讨Apicidin对体外培养人舌鳞癌CAL-27细胞影响及作用机制。方法体外培养人舌鳞癌CAL-27细胞,采用不同浓度的Apicidin作为实验组,并设立对照组,倒置显微镜观察细胞形态,CCK-8法检测细胞增殖、TUNEL、流式细胞仪检测Apicidin对CAL-27细胞凋亡作用。结果 Apicidin可显著抑制CAL-27细胞的生长(P<0.05),呈时间剂量依赖性。通过TUNEL法、流式细胞仪检测显示CAL-27细胞的凋亡,并且使CAL-27细胞停留在G2期。结论 Apicidin能显著抑制CAL-27的体外生长并能诱导细胞凋亡。
Objective To investigate the effects of Apicidin on human tongue squamous carcinoma cell line CAL-27 in vitro.Methods The human tongue squamous carcinoma CAL-27 cells were cultured regularly in medium with different concentration rations of Apicidin.Morphology of cells was examined by light microsope,proliferative activity of cells was assessed by CCK-8,the cell apoptosis was detected by TUNEL and FCM.Results A time dose-dependent significantly inhibited was detected in CAL-27 cells by Apicidin（P0.05）.Apicidin could induce the cell apoptosis of CAL-27 which revealed by TUNEL and FCM,and increase the proportion in G2/M phases of the cell cycle.Conclusion Apicidin significantly inhibited the growth of CAL-27 in vitro and can induce apoptosis.
出处
《现代口腔医学杂志》
CAS
CSCD
2012年第2期117-119,共3页
Journal of Modern Stomatology
基金
黑龙江省教育厅科学技术研究项目(11551188)
