摘要
目的建立检测内氏放线菌(Actinomyces naeslundii,An)与龋齿放线菌(Actinomyces odontolyticus,Ao)的实时荧光定量PCR(RT-PCR)。方法探讨An和Ao在唾液中的定植数量与乳牙龋失补牙面指数(dmfs)的关系。方法分别采集59名不同患龋状况儿童唾液样本,其中无龋组(dmfs=0)19名、中龋组(dmfs=4～6)21名和高龋组(dmfs﹥8)19名。提取细菌总基因组DNA,应用SYBR GreenⅡ模式的实时荧光定量PCR技术,对唾液中的内氏放线菌及龋齿放线菌进行定量检测,所得数据应用SAS9.2统计软件进行统计学分析。结果内氏放线菌占总菌的比例在三组中有显著差异(P<0.05),在高龋组中显著高于中龋组(P<0.0001),在中龋组与无龋组间无显著差异(P>0.05);龋齿放线菌占总菌的比例在三组中无显著差异(P>0.05)。结论儿童唾液中内氏放线菌的数量与龋病的发生发展过程有明显相关性;龋齿放线菌与儿童龋病的发生发展无明显相关性。
Objective This study aims to establish a RT-PCR method to detect Actinomyces naeslundii（An） and Actinomyces odontolyticus（Ao） and inquire into the relationship between Colonization quantity of An and Ao in saliva and dmfs.Methods We take 59 Cary Children＇s saliva samples,including 19 caries-free individuals（dmfs= zero）,21 mid-caries individuals（dmfs=4~6） and 19 caries-active individuals（dmfs8）.We isolate genomic DNA from bacterium,apply RT-PCR technology of SYBR Green Ⅱto detect An and Ao in saliva and use SAS9.2 software to carry out the data processing and analyses.Results The percentage of An in three groups is quite different（P0.05）,which is much higher in caries-active individuals than that in mid-caries individuals（P0.0001） and no significant difference between mid-caries and caries-free individuals（P0.05）;The percentage of Ao in three groups is not significantly different（P0.05）.Conclusion There is significant correlation between the amount of An in children＇s saliva and the onset and development of caries;and there is no significant correlation between the amount of Ao in children＇s saliva and the onset and development of caries.
出处
《现代口腔医学杂志》
CAS
CSCD
2012年第2期76-79,共4页
Journal of Modern Stomatology
