摘要
目的:了解我国登革病毒(Dengue virus,DV)包膜蛋白基因(Envelope,E)重组的发生情况。方法:从GenBank收集共70个DV中国株的E基因序列,Clustal-W比对后,重组检测软件包RDP3检测E基因中可能存在的重组事件,并经Mega4构建系统发生树验证所检测的重组事件的可靠性和真实性。结果:DV-1型中E基因区存在3个重组事件,共4个重组子,分别是1995、1997、1999年和2004年分离的DV病毒株,在其它3个血清型的DV病毒株中没有检测到重组,也未检测到血清型间重组。结论:我国DV 1型血清型E基因区域存在基因重组。
Objective：To explore the recombination of envelope protein（E） gene of Chinese dengue virus（DV） isolates.Methods：70 Chinese DV E gene sequences available from GenBank were collected,and aligned with Clustal-W,then were incorporated into recombination detection program（RDP3） for potential recombination event detection.Phylogenetic trees constructed by Mega4 were further generated to confirm the recombination events.Results：Three intra-serotype recombination events,four recombinants isolated in 1995,1997,1999,and 2004,respectively,were confirmed in DV-1 E gene data.However,no recombination event was detected in other three serotypes in the data,so was the inter-serotype recombination.Conclusion：Recombination of E gene regions of Chinese dengue virus type 1 exists,although limited data were available.It highlights the necessity of employing more gene regions and sequences in the analysis to fully detect the recombination in China.
出处
《重庆医科大学学报》
CAS
CSCD
北大核心
2012年第1期67-70,共4页
Journal of Chongqing Medical University
基金
重庆医科大学院青年教师科研基金资助项目(编号:0800280002)
关键词
RDP3
登革热
E基因
遗传重组
RDP3
dengue fever
envelope protein gene
genetic recombination
