摘要
目的探讨幼年和成年Wistar大鼠脑干胶质瘤侵袭性的差异。方法 50只幼年和成年Wistar大鼠随机分4组,A组（n=15）和C组（n=15）分别将大鼠C6胶质瘤细胞注射到幼鼠和成鼠脑桥,B组（n=10）和D组（n=10）大鼠分别注射等体积生理盐水到幼鼠和成鼠脑桥。2周后MRI观察肿瘤生长情况并测量体积,常规行苏木精-伊红染色观察肿瘤组织形态学变化,免疫组织化学染色检测细胞侵袭相关因子基质金属蛋白酶2（MMP-2）、基质金属蛋白酶9（MMP-9）和β-catenin的表达。记录4组大鼠生存时间。结果 MRI检查结果：A、C组在脑桥均发现肿瘤,肿瘤平均体积分别为（49.63±8.34）mm3和（52.07±7.77）mm3,两者无统计学差异（P〉0.05）;B、D组无肿瘤生长。A、C组大鼠平均生存时间分别为（19.47±2.23）d和（21.47±2.23）d,统计学比较有显著性差异（P〈0.05）;B、D组大鼠至另2组大鼠全部死亡时仍存活。A、C组肿瘤形态观察及MMP-2、MMP-9、β-catenin的表达无明显差异（均P〉0.05）。结论成功建立幼年和成年大鼠脑干胶质瘤动物模型,但二者肿瘤侵袭性无明显差异。
Taking a high-chromium steel as substrate, WC particles as enhanced particles, using the way of the V-EPC infiltration, which were used to make WC-particle reinforced steel-based metal matrix composite. The effects of thickness and microstmcture on relative thickness of the composite material were investigated. The microstructure of surface composite was observed by SEM, EDS and OM. The results show that using V-EPC can prepare WC particle reinforced steel-based surface composite. With the increase in the relative thickness of WC particle reinforced steel matrix composite, the thickness of transition layer increases, and the compound layer thickness decreases, then WC particle and the bonding strength increase.
出处
《热加工工艺》
CSCD
北大核心
2012年第6期82-84,共3页
Hot Working Technology
基金
安徽建筑工业学院青年专项经费基金资助（2011）
关键词
神经胶质瘤
脑干
侵袭性
大鼠
relative thickness
V-EPC
WC particle
surface composite
