摘要
目的 观察表皮生长因子 (EGF)对接受长期全肠外营养 (TPN)的腹部辐射大鼠肠道细菌移位的影响 ,探讨其机制 .方法 应用 TSB培养、图像分析、高效液相色谱等方法检测应用 EGF后大鼠死亡率 ,肠粘膜形态结构 ,肠道细菌移位 ,血浆及组织谷氨酰胺 (Gln)水平及肠道 Gln摄取率 .结果 应用 EGF后 ,大鼠死亡率显著降低 ,肠粘膜结构屏障改善 ,细菌移位减少 ,血液及组织 Gln水平提高 .结论 EGF在长期TPN显著改善辐射性肠粘膜屏障损伤大鼠营养的同时 ,减少肠道细菌移位 ,显著改善血液及组织的 Gln代谢 ,维持肠粘膜上皮的正常功能 ,加速修复损伤的肠粘膜屏障 ,降低死亡率 .
AIM To investigate the effects of EGF on bacterial translocation from gut in parenterally fed rats following abdominal radiation and to study its mechanism. METHODS The TSB culture, image processing and analysis system, and HPLC were employed for the morphological evaluation of gut mucosa, bacterial translocation from gut, glutamine content in blood and tissue, and gut glutamine extraction. RESULTS When EGF was administrated along with TPN in the rat following abdominal radiation, EGF could increase the glutamine content in blood and tissue and gut glutamine extraction significantly, improve gut mucosa barrier function, decrease the bacterial translocation from gut and decrease mortality. CONCLUSION While TPN offered the abdominal radiation rats an satisfactory nutrition, the EGF can increase the gut glutamine extraction and utilization and improve gut mucosa barrier function. Thus the mortality of TPN fed rats following abdominal radiation can be reduced.
出处
《第四军医大学学报》
2000年第1期76-79,共4页
Journal of the Fourth Military Medical University
关键词
腹部辐射
表皮生长因子
胃肠外营养
细菌移位
abdominal radiation
epidermal growth factor
total parenteral nutrition
bacterial translocation
