摘要
目的探讨溃疡性结肠炎(UC)患者肠黏膜活检组织中Toll样受体4(TLR4)、核转录因子-κB(NF-κBp65)、白介素-8(IL-8)的表达及三者在UC发病机制中的作用。方法 内镜活检UC活动期结肠黏膜标本68份(UC组)及30例正常人结肠黏膜标本(健康对照组),采用免疫组化SP方法检测TLR4、NF-κB p65、IL-8的表达水平,并做统计学处理。结果 UC组结肠黏膜表面上皮细胞和固有层TLR4、NF-κB p65、IL-8呈阳性表达,染色为深棕黄色,较健康对照组表达均显著增强,且不同病理分级间比较,Ⅲ级>Ⅱ级>Ⅰ级,差异均有统计学意义(p<0.05)。结论 UC肠黏膜中TLR4、NF-κB p65、IL-8的表达均高度上调,并随UC病理分级的升高而升高。
Objective To characterize the difference of the expression of the TLR4,NF-κB p65 and IL-8 protein in ulcerative colitis（UC）,to explore the possible effects of TLR4,NF-κB p65 and IL-8 in the imitation and progression of UC. Methods Colonic biopsy specimens were collected from active UC（n =68） and normal controls（n =30）.The expression of TLR4,NF-κB p65 and IL-8 were analyzed with immunohistochemistry（IHC）.Results The expression of the TLR4,NF-κB p65 and IL-8 protein was significantly increased in epithelial cells and the lamina propria of UC compared with controls. The TLR4,IL-8 and NF-κB p65 protein were positively related to pathological classification in UC.They are significantly higher in classⅡand classⅢthan that in classⅠ（P 0.05） as well as in classⅢthan in classⅡ（P 0.05）.Conclusion In UC,the expression of the TLR4,IL-8 and NF-κB p65 protein were up-regulated significantly,and it was closely associated with the course of ulcerative colitis.
出处
《疑难病杂志》
CAS
2012年第3期181-183,F0003,共4页
Chinese Journal of Difficult and Complicated Cases
基金
河北省卫生厅医学科学研究重点课题(No.20090099)
