摘要
以768只中国美利奴羊(新疆军垦型)为材料,采用测序和PCR技术分析KAP1.1(B2A)基因单核苷酸多态性及其与羊毛纤维直径的相关性。结果表明:中国美利奴羊(新疆军垦型)KAP1.1基因存在30个碱基的插入/缺失和6种基因型,分别为(341 bp)AA基因型、(311 bp)BB基因型、(281 bp)CC基因型、(341 bp/311 bp)AB基因型、(341 bp/281 bp)AC基因型和(311 bp/281 bp)BC基因型。AA、BC、AC、AB和CC基因型间的毛卷曲度、细度离散、毛长、污毛重、净毛率和密度差异不显著(P>0.05),而BB基因型羊只的平均纤维直径显著小于AA、BC、AC、AB和CC基因型羊只的平均纤维直径(P<0.05)。以上结果表明,KAP1.1基因可作为绵羊毛纤维直径的主要候选基因之一,BB基因型可作为分子标记用于预测绵羊毛纤维直径。
This study used 768 Chinese Merino（ XinJiang JunKen Type）. Sequencing and PCR technology were used to analysis KAP1.1 （B2A）gene polymorphisms and association of KAP1.1 gene with wool fiber diameter. The results showed six genotypes and 30 bp insertion/deletion of KAP1.1 gene were existed in Chinese Merino（ XinJiang Junken Type）, which were AA genotype （ 341 bp ）, BB genotype （ 311 bp）, CC genotype （ 281 bp）, AB genotype （341 hp/311 hp）,AC genotype（341 hp/281 hp） and BC genotype（311 bp/281 bp）,respectively. Least square linear model（LSM）analysis indicated that the genotypes AA, BC, AC, AB and CC were not associated with wool curvature, Fineness Dispersion coefficient, staple length, fleece weight, clean fleece weight, wool density （P 〉 0. 05 ）. Genotype BB showed significantly less than BC, AA, AC ,AB and CC genotypes in wool fiber diameter（P 〈 0. 05 ）. KAP1.1 gene was proved to be closely related to wool fiber diameter,which can be used as a candidate mo- lecular marker for wool fiber diameter in sheep.
出处
《华北农学报》
CSCD
北大核心
2012年第1期45-50,共6页
Acta Agriculturae Boreali-Sinica
基金
转基因生物新品种培育科技重大专项(2009ZX08009-160B)
新疆农垦科学院科技引导计划(YYD2010-8)
