期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

利用mRNA差异显示技术筛选绵羊毛性状相关基因 被引量：1

Isolation of Genes Associated with Wool Traits by mRNA Differential Display
在线阅读 下载PDF
导出
摘要 利用mRNA差异显示技术(DD-PCR),比较绵羊肩、腹、腿三部位皮肤中mRNA的差异表达,共获得差异表达的cDNA片段16条,其中肩部、腹部与腿部之间的差异较大;对所有16条差异片段进行测序并在GENBANK中进行检索,未发现有同源序列。提示16条cDNA片段可能为未发现的基因片段,也可能存在假阳性片段。 mRNA expression in different parts of sheep skin（ shoulder, abdomen and midleg）were compared by mRNA differential display technique. 16 differential cDNA fragments were obtained. Among them, differences between shoulder and mid-leg were maximum. Then their sequences were determined and blasted in GENBANK, but homologous sequences were not detected. So 16 cDNA fragmnents may be sequences, but had been not found or some of them might be false positive fragments. The results lay basisfor studies on genes associated with wool traits and functions of these genes.
作者 牟艳军 刘国龄 廖和荣 沙莎 赵宗胜 李大全
机构地区 石河子大学动物科技学院 新疆自治区畜牧厅畜禽改良总站
出处 《石河子大学学报（自然科学版）》 CAS 2006年第1期95-97,共3页 Journal of Shihezi University(Natural Science)
关键词 绵羊 皮肤 羊毛性状 MRNA差异显示技术 sheep skin wool traits mRNA differential display
分类号 S826.2 [农业科学—畜牧学]
  • 相关文献

参考文献10

  • 1Liang P.Differential display of eukaryotic messenger RNA by means of the polymerase chain reaction[J].Science,1992,257:967-971.
  • 2Carl W,Dieffenbach,Gabriela S,et al.PCR Primer:A Laboratory Manual(2nd edition)[M].北京:科学出版社,2004:327-348
  • 3Offensperger W B,Blum H E,Gerokw.Therapy of hepadnavirus infection using antisense oligonucleotides[J].Intervirology,1995,38(2):113-119.
  • 4Sui G,Soohoo C,Affarel B,et al.A DNA vector-based RNAi technology to suppress gene expression in mammalian cells[J].Proc Natl Acad Sci USA,2002,99:5515-5552.
  • 5Budowle B.Analysis of the VNTR locus DIS80 by the PCR followed by highresolution PAGE[J].AM Jhum Genet,1991,48:137.
  • 6Bassam B J.Fast and sensitive silver staining of DNA in polyacrylamide gels[J].Anal Biochem,1991,196:80.
  • 7Southern E M,Case Green S C,Elder J K,et al.Arrays of complementary oligonucleotides for analyzing the hybridisation behaviour of nucleic acids[J].Ncleic Acids Res,1994,22:1368-1373.
  • 8Ramsay G.DNA chips:state-of-the-art[J].Nature Botech,1998,16:40-44.
  • 9Velculescu F B,Zhang L,Vogelstein B,et al.Serial analysis of gene expression[J].Science,1995,270:484-4872.
  • 10Liang P,Bauer D,Averboukh L,et al.Anlysis of altered gene expression by differential display[J].Methods Enzymol,1995,254:304-321.

同被引文献30

  • 1李长青,尹俊,张燕军,郭志成,张文广,高爱琴,李玉荣,赖双英,李金泉.内蒙古绒山羊与辽宁绒山羊皮肤毛囊周期性变化的比较研究[J].畜牧兽医学报,2005,36(7):674-679. 被引量：54
  • 2McLaren R J, Rogers G R, Davies K P, Maddox J F, Montgomery G W. Linkage mapping of wool keratin and keratin-associated protein genes in sheep. Mammalian Genome, 1997, 8(12): 938-940.
  • 3Cockett N E, Shay T L, Smit M. Analysis of the sheep gcnome. Physiological Genomics, 2001, 7(2): 69-78.
  • 4Adelson D L, Cam G R, DeSilva U, Franklin I R. Gene expression in sheep skin and wool (hair). Genomics, 2004, 83(1): 95-105.
  • 5李玉荣,尹俊,张燕军,刘羿羿期皮肤毛囊差异表达基因研究146-148.李金泉.绒山羊胚胎期和兴盛湖北农业科学,2008,47(2):.
  • 6Xie W, Li F, Kudlow J E, Wu C. Expression of the integrin-linked kinase (ILK) in mouse kin: loss of expression in suprabasal layers of the epidermis and up-regulation by erbB-2. American Journal of Pathology, 1998, 153(2): 367-372.
  • 7Stenn K S, Paus R. Controls of hair follicle cycling. Physiology Reviews, 2001, 81: 449-494.
  • 8Moll R, Divo M, Langbein L. The human keratins: biology andpathology. Histochemistry and Cell Biology, 2008, 129(6): 705-733.
  • 9Yu Z D, Gordon S W, Nixon A J, Bawden C S, Rogers M A, Wildermoth J E, Maqbool N J, Pearson A J. Expression patterns of keratin intermediate filament and keratin associated protein genes in wool follicles. Differentiation, 2009, 77(3): 307-316.
  • 10Whitbread L R Powell B C. Expression of the intermediate filament keratin gene, K15, in the basal cell layers of epithelia and the hair follicle. Experimental Cell Research, 1998, 244: 448-459.

引证文献1

二级引证文献3

石河子大学学报（自然科学版）
2006年 第1期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部