摘要
目的:提取血链球菌胞内蛋白与胞外蛋白,研究两者对中间普氏菌(P.i)与牙龈卟啉单胞菌(P.g)生物膜的作用。方法:通过低温超速离心,有机溶剂萃取法提取血链球菌胞外蛋白;通过超声破碎,硫酸铵盐析,SephadexG-25除盐及透析的方法提取血链球菌胞内蛋白。观察血链球菌胞内蛋白与胞外蛋白对P.i及P.g的作用。观察血链球菌胞内蛋白与胞外蛋白对P.i及P.g混合培养形成的生物膜作用。结果:血链球菌胞内蛋白对混合培养的P.i与P.g的生长有明显抑制作用,其最小抑菌浓度为0.125g/L;血链球菌胞外蛋白对P.i与P.g未见抑菌作用。P.i与P.g混合培养形成的生物膜在血链球菌胞内蛋白作用后,生物膜活性降低,与对照组相比具有显著差异(P<0.05);但在血链球菌胞外蛋白作用后,生物膜活性与对照组相比差异不具有统计学意义。结论:血链球菌胞内蛋白对P.i和P.g及两者混合培养形成的生物膜具有明显抑制作用;而血链球菌胞外蛋白对P.i和P.g及两者混合培养形成的生物膜无抑制作用。
Objective： To investigate the effects of Prevotella intermedia（P, i） and Porphyromonas gingivalis（P, g） in biofilms by extract extracellular and intracellular proteins of Streptococcus sanguis（S, s）. Methods： Extract extracellular proteins of S. s by high speed centrifugation using organic solvent; by ultrasonic, ammonium sulfate salting out, Sephadex G-25 column desalting through dialysis to extract intracellular proteins. Observe the effects of the intracellular proteins and extracellular proteins of S. s on biofilms of P. i and P. g. Results： The growth of P. i and P. g is significantly inhibited by intracellular proteins of S. s. The MIC of intracellular proteins on mixed P. i and P. g is 0. 125g/L;there are no significant effect of extracellular proteins of S. s on P. i and P.g. Intracellular proteins of S. s role in the biofilms of P. i and P. g mixed,the biofilms activity was decreased,compared with the control group have significant differences（P〈0.05） ; however, which roled of the extracellular proteins of S. s,the difference was not statistically significant which on the activity of biofilm compared with the control group. Conclusion： P. i, P. g and the mixed bacteria in biofilms which roled of intracellular proteins of S. s have significantly inhibition effect; there are no significantly inhibition effect when the extracellular proteins role of the P. i, P. g and the mixed bacteria in biofilms
出处
《口腔医学研究》
CAS
CSCD
2012年第3期206-209,共4页
Journal of Oral Science Research
基金
黑龙江省自然科学基金资助项目(D2007-03)
