摘要
目的:通过对牙龈卟啉单胞菌(Porphyromonas gingivalis,P.gingivalis)PG0839基因突变菌株的生物学特性和毒力进行研究,为进一步明确毒力岛基因PG0839的功能提供实验依据。方法:培养P.gingivalis W83和PG0839基因突变菌株,观察2个菌株菌落形态特征和革兰染色镜下特征,绘制菌株的生长曲线。制备1%绵羊红细胞,取2种细菌细胞,进行绵羊红细胞凝集活性检测。分别接种浓度为2×1010、1×1010、5×109 CFU/ml的P.gingivalis W83和PG0839基因突变株菌液于小鼠背部。采用SPSS 13.0软件包中的皮尔森卡方检验和对数秩检验对不同组别小鼠生存情况进行分析。结果:与P.gingivalis W83比较,PG0839突变菌株产生黑色素和红细胞凝集活性显著降低。2个菌株生长曲线未见显著差异(U=25.50,P=0.19)。小鼠皮下感染模型表明,PG0839基因突变菌株毒性显著低于W83菌株(P<0.05)。结论:PG0839基因的缺失改变了细菌的一些生物学特征,提示PG0839基因可能调控P.gingivalis某些基因或者基因产物的表达。同时,PG0839基因在P.gingivalis致病过程中发挥一定作用。
PURPOSE： To study the effect of PG0839 gene form Porphyromonas gingivalis（P.gingivalis） on biological characterization and virulence in a murine model of soft tissue destruction,and further to provide evidence for clarifying the gene function of PG0839.METHODS： P.gingivalis W83 and PG0839-defective mutant strains were plated on brain heart infusion（BHI）.Colonial formation,micromorphology and growth curve of the two strains were observed.The two strains cells were mixed respectively with sheep erythrocytes,which were prepared to a final concentration of 1% in 1×PBS,to assess hemagglutination activity.The mice were challenged with subcutaneous injections of bacterial suspension,which were P.gingivalis W83 and PG0839-defective mutant strains at the doses of 2×1010,1×1010,5×109 CFU/mL respectively,on the dorsal surface.Difference in survival situation of mice in various groups was examined using Pearson Chi-square and log rank test with SPSS 13.0 software package.RESULTS： In contrast to the wild-type W83 strain,PG0839-defective mutant strain was nonpigmented and the hemagglutinin activities reduced.However,the growth rate of PG0839-defective mutant strain was not affected（U=25.50,P=0.19）.Results of the mice subcutaneous infection model indicated that the virulence of the PG0839-defective mutant strain was significantly lower than P.gingivalis W83 strain（P0.05）.CONCLUSIONS： Absence of PG0839 gene altered several biological characteristics of P.gingivalis W83 strain,suggesting that PG0839 gene may regulate the expression of certain gene or gene product of P.gingivalis W83 strain.Moreover,PG0839 gene may contribute to the virulence of P.gingivalis W83.
出处
《上海口腔医学》
CAS
CSCD
2011年第5期449-453,共5页
Shanghai Journal of Stomatology
基金
国家自然科学基金(81070834)~~
关键词
牙龈卟啉单胞菌
毒力岛
红细胞凝集活性
生存率
Porphyromonas gingivalis
Pathogenicity islands
Hemagglutination activity
Survival rate
