摘要
目的研究决明子提取物对溴酸钾(KBrO3)/伤寒沙门氏菌脂多糖(LPS)诱发小鼠晶状体氧化应激状态的改善作用。方法小鼠尾静脉注射2 mg.kg-1LPS,同时腹腔注射200 mg.kg-1KBrO3制备小鼠晶状体氧化应激模型。50,150和450 mg.kg-1决明子提取物在LPS/KBrO3给药同时及3 h前分别灌胃2次,对照组灌胃同体积水溶液。LPS/KBrO3给药6 h后,乙醚麻醉条件下采取小鼠晶状体测定抗氧化能力指标。结果单独腹腔注射200 mg.kg-1KBrO3或尾静脉注射2 mg.kg-1LPS 6 h后,小鼠晶状体组织内谷胱甘肽(GSH)水平均有所降低,而当KBrO3并用LPS时则显著诱发小鼠晶状体氧化应激状态。50,150及450 mg.kg-1决明子提取物灌胃给药能够有效的改善因KBrO3/LPS诱导小鼠晶状体组织内GSH含量的减少、提高抗氧化能力指数(ORAC)和降低丙二醛(MDA)水平,并呈一定的量效关系。结论决明子提取物可以改善小鼠晶状体内的过氧化状态,其作用机制可能是通过清除自由基和抑制脂质过氧化过程实现的。
Objective To investigate the protective effects of Semen Cassiae extract （SCE） on oxidative status of mice lens induced by kalium bromicum/typhoid Bacillus lipopolysaccharide （KBrO3/LPS）. Methods The mice lens oxida- tive stress model was established by injection of LPS （2 mg·kg-1） into the caudal vein and synchronously intraperi toneal injection of KBrO3（200 rng＇kg-1） in Kuming mice. Low, moderate and high dose of SCE（50, 150 and 450 mg· kg-1） were given intragastrically to mice for twice, 3 hours early before and at the same time of KBrO3/LPS administration, respectively. The control groups were given the same volume of water. Mice lens were taken out 6 hours after KBrO3/LPS injection to evaluate antioxidant capacity indexes. Results Intraperitoneal injection of KBrO3（200 mg＇kg-1） or injection of LPS（2 mg＇kg-1） into the caudal vein alone decreased glutathione（GSH） content in mice lens 6 hours after administration, but the combination of KBrO3 and LPS induced the oxidative stress status of mice lens. The administration of SCE could effectively improve the reduction of GSH content, elevate the oxygen-radical absorb ing capality（ORAC） and decrease MDA content, the effect was in dose-dependent manner. Conclusion SCE could improve oxidative stress status of mice lens induced by KBrO3/LPS. The mechanism may be related to the elimination of free radicals and inhibition of lipid peroxidation.
出处
《中药新药与临床药理》
CAS
CSCD
北大核心
2012年第2期167-170,共4页
Traditional Chinese Drug Research and Clinical Pharmacology
关键词
决明子
晶状体
溴酸钾
内毒素
氧化应激损伤
Semen Cassiae extract
Lens
Kalium Bromicum
Endotoxin
Oxidative Damage
