摘要
目的研究虎眼万年青总皂苷对人乳腺癌MDA-MB-231细胞增殖和凋亡的影响。方法用MTT比色法检测虎眼万年青总皂苷对MDA-MB-231细胞的增殖抑制作用;流式细胞术分析细胞凋亡与细胞周期变化;透射电镜观察细胞形态学变化。结果虎眼万年青总皂苷对MDA-MB-231细胞生长有明显抑制作用,并呈剂量、时间依赖性;可诱导细胞发生凋亡,且凋亡率呈浓度依赖性,使细胞周期明显阻滞于G0/G1期;电镜下可见典型的细胞凋亡形态学改变。结论虎眼万年青总皂苷可抑制人乳腺癌MDA-MB-231细胞增殖,并可诱导细胞凋亡,其机制可能与虎眼万年青总皂苷将癌细胞阻止于G0/G1期相关。
Objective To investigate the effects of Ornithogalum caudatum Ait saponins on the proliferation and apop tosis of breast cancer cell line MDA-MB-231. Methods MTF assay was used to measure the antiproliferative effect of Ornithogalum caudatum Ait saponins. The apoptotic rate and the cell cycle were analyzed by flow cytometry, and the morphological changes of cells were observed under transmission electron microscope. Results Ornithogalum cauda- tum Ait saponins inhibited the proliferation of MDA-MB-231 cells in dose and timedependent manner. Ornithogalum caudatum Ait saponins induced the apoptosis of cells in dosedependent manner. The growth of cells was arrested in Go/G1 phase. Typical apoptotic morphological changes of cells were found under transmission electron microscope. Conclusion Ornithogalum caudatum Ait saponins inhibit the proliferation of MDA-MB-231 cells and induce apopto sis, and the possible mechanism is probably related with blocking the growth of cells in GO/G1 phase.
出处
《中药新药与临床药理》
CAS
CSCD
北大核心
2012年第2期121-124,共4页
Traditional Chinese Drug Research and Clinical Pharmacology
基金
江苏省自然科学基金项目(BK2002201)
