摘要
目的探讨人乳头状瘤病毒(HPV)感染和p53基因多态性与食管癌的关系。方法收集温岭市食管癌患者204例与健康对照组102例,取活组织标本提取基因组,对提取的DNA样品,用PCR方法检测HPV,PCR-RFLP方法分析p53基因第72密码子多态性,对照分析其与食管癌发生率的关系。结果 PCR检测结果显示,食管癌组和对照组患者HPV16检出率分别为59.3%及11.8%,食管癌患者和正常对照组p53基因72密码子Arg/Arg基因型频率,分别为51.5%及19.6%,两组对比差异均有统计学意义(P<0.05);所有食管癌患者中,p53 Arg/Arg基因型在HPV16阳性组中占66.9%,在阴性组中占28.9%,两组对比差异有统计学意义(P<0.05);Arg/Pro和Pro/Pro基因型在HPV16阳性中占33.1%,在HPV16阴性中占72.1%,差异有统计学意义(P<0.05)。结论高危型HPV16感染可能是温岭市食管癌高发区的危险因素,Arg/Arg基因型可能是该地区食管癌患者的易感基因型,个体携带p53 Arg/Arg基因型更容易发生HPV相关的食管癌。
OBJECTIVE To study the relationship between HPV infections, p53 polymorphisms and esophageal cancer. METHODS A case-control study was performed in 204 esophageal cancer patients and I02 healthy controls in Wenling city. DNA samples were extracted from living tissues and the HPV were detected by PCR. The relationship between polymorphism codon 72 of p53 and the esolhageal cancer incidence rate was analyzed. RESULTS The positive rate of HPV16 in esophageal cancer group （59.3%） in Wenting was higher than that in control group （11.8%）, （P〈0.05）. And the frequency of Arg/Arg genotype on codon 72 of p53 in esolhageal cancer group （51.5% ） was higher than that in control group （19.6%）, the difference was statistically significant （P〈0.05）. The frequency of Arg/Arg genotype in HPV16 positive group （66. 9%） was higher than that in negative group （28. 9%）, the difference was statistically significant. The account of Arg/Pro and Pro/Pro genotype was 33. 1% in HPV16 positive groups and 72. 1% in negative group the difference was seatistically signifiant （P〈 0. 05）. CONCLUSION High risk HPV plays an important role in development of esophageal cancer, p53 Arg/Arg genotype is one of the high risk genetic factors for HPV-associated espphageal cancer in Wenling.
出处
《中华医院感染学杂志》
CAS
CSCD
北大核心
2012年第5期910-912,共3页
Chinese Journal of Nosocomiology
基金
温岭市科技局基金资助项目(2009-58)
