摘要
为获得聚-β-羟基丁酸(PHB)提取的最佳工艺,以氯仿浓度,提取时间,和提取温度为影响因子,在单因素实验结果的基础上,应用Box-Benhnken中心组合方法进行三因素三水平的实验设计,以PHB提取量为响应值,运用响应面法(RSM)对提取条件进行进一步的优化。结果表明:在氯仿浓度69.7%,提取温度59.7℃,提取时间3.0 h的条件下,PHB提取量达到最大值4.430 g/L。
To obtain the optimal extraction conditions of Poly-β-hydroxybutyrate （PHB） in Bacillus megaterium, the effect of chloroform concentration, extraction time and temperature on PHB extraction were studied. On the basis of single factor experiment, Box-Benhnken central composite method was designed for the three factors. In response to PHB extrac- tion value, the extraction conditions were further optimized by response surface method （RSM）. The results showed that when chloroform concentration was 69.7% ,extraction temperature was 59.7 ~C ,extraction time was 3.0 h,the produc- tivity of PHB was 4.437 g/L, reached its maximum.
出处
《天然产物研究与开发》
CAS
CSCD
北大核心
2012年第3期393-397,共5页
Natural Product Research and Development
基金
浙江省公益性技术应用研究计划项目(2010C32076)
浙江省自然科学基金项目(Y3090343)
浙江省大学生科技创新活动计划(2010R404064)
