摘要
目的:建立傣药蓬莱葛中砷和汞的原子荧光光谱检测方法。方法:采用氢化物发生-原子荧光分光光度法测定蓬莱葛中砷、汞的含量,并对测定方法进行方法学考察。结果:在最佳工作条件下,砷、汞的浓度与荧光强度均呈现良好的线性关系。砷的检测限为0.0148μg.L-1,RSD为≤4.0%,平均回收率为108.6%～109.7%;汞的检测限为0.1046μg.L-1,RSD≤5.7%,平均回收率为94.4%～109.0%。蓬莱葛中砷和汞元素平均含量分别为0.1537μg.g-1、0.0391μg.g-1。傣药蓬莱葛中砷、汞有害元素含量符合2010年版中国药典中有害元素的限量标准。结论:本文所用方法快速、简便、准确且灵敏度高、效果满意,为蓬莱葛中重金属含量测定提供了较好的方法。
Objective：To establish a quantity determination method of atomic fluorescence spectrometry for detecting arsenic（As）and mercury（Hg）in Gardneria multiflora Makino.Methods：The atomic fluorescence spectrometry was adopted to determine the contents of As and Hg in Gardneria multiflora Makino,and the determination method was studied by methodology.Results：Under the optimized experimental conditions,the concentration of As and Hg showed a linear relationship with the fluorescence intensity.The detection limit of As was 0.0148μg·L-1,and the RSD were less than 4.0%,and the average recoveries were in the range of 108.6%-109.7%.The detection limit of Hg was 0.1046μg·L-1,and the RSD were less than 5.7%,and the average recoveries were in the range of 94.4%-109.0%.The average contents of As and Hg in Gardneria multiflora Makino were 0.1537μg·g-1 and 0.0391μg·g-1.The contents of the above As and Hg in Gardneria multiflora Makino were accorded with the quantity limitation standard of ChP（edition 2010）.Conclusion：The method is easy,quick,accurate with a higher sensitivity,which can be used in the quantitative determination of heavy metal elements in Gardneria multiflora Makino with a satisfactory result.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2012年第3期443-446,共4页
Chinese Journal of Pharmaceutical Analysis
关键词
民族药
广蒿修(蓬莱葛)
砷
汞
有害元素
原子荧光光谱法
安全监测
ethnic drug
Gardneria multiflora Makino
arsenic
mercury
harmful element
atomic fluorescence spectrometry
safety monitoring
