摘要
目的用HPLC测定可乐中咖啡因的含量,建立测定可乐中咖啡因含量的高效液相色谱法的最佳工作条件。方法色谱柱采用kromasilC18柱(250mm×4.6mm,5μm);流动相:甲醇-水(40:60);流速:1.0ml/min;检测波长为275nm;进样量20μl;柱温为室温。结果咖啡因与其它杂质均能达到基线分离,其色谱峰形好。咖啡因的浓度在30～70μg/ml时与峰面积成良好的线性关系(y=27498x+67325,r=0.9994),平均加样回收率为100.1%,方法精密度RSD为1.2%(n=6)。结论该方法准确、简便、快速,适合可乐中咖啡因的含量测定。
Objective To determine the determination of caffeine in cola by high performance liquid chromatograph and optimize the HPLC separation conditions.Methods The chromatographic column kromasil C18（250mm×4.6mm,5μm） and Methanol/H2O（40：60） as mobile phase were used.The flow rate was 1.0ml/min and the detective wavelength was 275nm.Injection volume was 20μl.The column temperature was room temperature.Results Caffeine and other peaks were separated well and the peak shape was presented well.There was a good linearity within the range of 30 to 70μg/ml of caffeine.The average recovery was 101.3%.The RSD was 1.15%（n=6）.Conclusion This method is accurate,simple and quick.Therefore it is suitable for the determination of caffeine in cola.
出处
《济宁医学院学报》
2012年第2期106-108,共3页
Journal of Jining Medical University
关键词
高效液相色谱法
可乐
咖啡因
High performance liquid chromatograph Cola Caffeine
