摘要
目的:观察癫痫发生过程和低频率电刺激癫痫灶点对自发性神经病理性疼痛的作用。方法:在大鼠杏仁核植入双极电极,恒流电刺激制备电点燃癫痫模型。在癫痫进展的不同阶段(局灶性癫痫发作和全身性癫痫发作)行坐骨神经和隐神经切断术,制备自发性神经病理性疼痛模型,并对大鼠在神经损伤后63 d内的自噬行为进行等级评分。大鼠分为对照组(n=7),局灶性癫痫组(1～3级,n=5),全身性癫痫组(4～5级,n=7),局灶性癫痫加低频电刺激组(n=4),全身性癫痫加低频电刺激组(n=4)。低频电刺激施加在癫痫灶点杏仁核,从神经切断术后开始至术后第21天。结果:局灶性癫痫组的大鼠自噬行为等级在术后第40～63天显著低于对照组,等级进展的曲线下面积由对照组的308.2±51.57下降到45.80±24.64,自噬发生时间比对照组延迟约32 d,没有1例动物在术后第63天表现出高分值自噬行为,而对照组的高分值发生率为71.4%;全身性癫痫组的大鼠自噬行为与对照组相比仅在开始时间上有明显差异,延迟约16 d;不论是局灶性癫痫还是全身性癫痫的大鼠,在其杏仁核施加低频电刺激后,自噬行为的进展和严重程度均与对照组相似。结论:局灶性癫痫大鼠对神经病理性疼痛的敏感性显著降低,低频电刺激杏仁核可以抑制这种变化。
Objective： To investigate the effects of epileptogenesis and low frequency stimulation at epileptic focus on spontaneous neuropathic pain in rats. Methods： Bipolar stimulating electrodes were implanted in the amygdala and current with constant intensity was applied to evoke kindling-induced seizures.In partial and generalized stages of seizure acquisition,neuroma model of spontaneous neuropathic pain was prepared by completely transection of the left sciatic and saphenous nerves of rats.Autotomy behavior was scored daily until d 63 postoperatively.Rats were divided into 5 groups：Control（n=7）,rats with partial seizures（1-3 stages,n=5）,rats with generalized seizures（4-5 stages,n=7）,rats with partial seizures and low frequency stimulation（n=4）,rats with generalized seizures and low frequency stimulation（n=4）.Low frequency stimulation was applied to the amygdala,the epileptic focus for 21 d from the d 2 after nerve transection. Results： Autotomy level in rats with partial seizures was significantly lower than that in controls.The autotomy scores during postoperative d 40~63 were significantly lower than those of controls,the area under the progression curve of autotomy behavior was decreased from 308.2±51.57 to 45.80±24.64,the onset day of autotomy was postponed by 32 d and none of the animals with partial seizures showed high autotomy,while 71.4% of controls showed that on d 63 postoperatively.Rats with generalized seizures showed autotomy similar to controls,except that the onset day was postponed by 16 d.Autotomy behavior in rats receiving low frequency stimulation of the amygdala was not different from that in controls. Conclusion： Focal seizures can lower sensitivity to spontaneous neuropathic pain in rats,while low frequency stimulation applied to the focus can abolish such effect.
出处
《浙江大学学报（医学版）》
CAS
CSCD
北大核心
2012年第1期47-53,共7页
Journal of Zhejiang University(Medical Sciences)
基金
国家杰出青年基金资助项目(30725047)
国家自然科学基金资助项目(30701015)
关键词
杏仁核/代谢
疾病模型
动物
电刺激
神经痛
癫痫
部分性
癫痫
Amygdala/metabolism
Disease models
animal
Electric stimulation
Neuralgia
Epilepsies
partial
Epilepsy
