摘要
目的:筛查中药材木香中潜在的肝毒性组分。方法:对木香25个化学组分,采用二乙酸荧光素荧光标记法在HepG2细胞模型上筛查肝毒性组分,并使用气相色谱-质谱联用仪分析其化学组成。结果:从木香中筛查出10个肝毒性组分,经气相色谱-质谱联用定性分析,C09组分的主要成分为去氢木香内酯、santamarine(或magnolialide)和reynosin,而C11组分的主要成分是α-木香醇和榄香醇。结论:木香所含的去氢木香内酯、santamarine(或magnolialide)、reynosin、α-木香醇和榄香醇可能具有肝毒性。
Objective： To screen the potential hepatotoxic components in Chinese herb medicine Aucklandiae Radix. Methods： The potential hepatotoxic components were screened using HepG2 cells labeled with fluorescein diacetate from 25 fractions of Aucklandiae Radix,in which the hepatotoxic compounds were further identified with GC-MS. Results： Ten potential hepatotoxic fractions were screened.The identification results by GC-MS indicated that the main compounds in C09 were dehydrocostuslactone,santamarine（or magnolialide） and reynosin,and in C11 were α-costol and elemol. Conclusion： Dehydrocostuslactone,santamarine（or magnolialide）,reynosin,α-costol and elemol are potential hepatotoxic compounds in Aucklandiae Radix.
出处
《浙江大学学报（医学版）》
CAS
CSCD
北大核心
2012年第1期43-46,共4页
Journal of Zhejiang University(Medical Sciences)
基金
浙江省科技计划项目(2008C23065)
关键词
气相色谱-质谱法
油类
挥发/分析
木香/分析
内酯类/分析
染色与标记
荧光素类
肝炎
中毒性
Gas chromatography-mass spectrometry
Oils
volatile/analysis
Aucklandiae Radix/analysis
Lactones/analysis
Staining and labeling
Fluoresceins
Hepatitis
toxic
