摘要
目的观察白蚁菌圃醇提液对α-突触核蛋白（a—synuclein）转基因帕金森病（PD）果蝇模型攀爬能力及寿命的影响以探讨其抗PD作用机制。方法采用乙醇提取白蚁菌圃，通过果蝇添食不同浓度白蚁菌圃醇提液（每100g培养液添加1、10和100mg），观察比较PD果蝇的攀爬能力、寿命；用刚果红染色观察脑组织病理改变。结果白蚁菌圃醇提取液提高PD果蝇攀爬能力，在其羽化后18～36d最显著，攀爬能力随白蚁菌圃醇提取液浓度升高及果蝇年龄的增长而增高。白蚁菌圃醇提取液延长PD果蝇的寿命雄果蝇寿命较PD果蝇对照延长31．82％～3．34％（均P〈0．05），并呈剂量依赖关系。白蚁菌圃醇提液作用后PD果蝇脑组织中的神经细胞数明显增加，空泡减少，且与PD果蝇的行为及寿命变化一致。结论白蚁菌圃醇提取液改善PD果蝇行为及延长PD果蝇寿命。
Objective To observe the effects of the extract of termitarium on the climbing ability and life span of Parkinson disease （PD）drosophila（Ddc-GAL4/＋ ;UAS-wild-type α- synuclein/＋ ）, and to explore its mechanism of anti-PD. Methods The termitarium was extracted by ethanol. PD drosophilas overexpressed α-synuclein were fed with 1,10 and 100 mg of termitarium per 100 g culture medium. The climbing ability and life span of drosophilas and pathological changes of its brain tissue （Congo red staining） were observed and compared among different concentrations. Results Termitarium enhanced the climbing ability of PD drosophilas and the most significant improvement appeared from 18 d to 36 d after eclosion, increasing with more termitarium concentrations and growing age of PD drosophilas. Termitarium also prolonged the life span among which male and female drosophila were longer 31.82%- 3.34%, 1.47%- 3.34% as compared with PD drosophila control （all P〈0.05）. The brain cells number in the PD drosophilas were increased and empty bubble was decreased by termitarium, accompanying with improvement of behavior and life span. Conclusions Termitarium can improve behavior and prolong life span of PD drosophilas.
出处
《中华老年医学杂志》
CAS
CSCD
北大核心
2012年第3期236-238,共3页
Chinese Journal of Geriatrics
关键词
白蚁
中草药
果蝇
帕金森病
预期寿命
行为
Termites
Drugs, Chinese herbal
Drosophilidae
Parkinson disease
Life expectancy
Behavior
