摘要
目的探讨大鼠腹主动脉球囊扩张术后内膜增生、钙调神经磷酸酶(calcineurin,CaN)信号通路以及炎症水平的变化。方法雄性SD大鼠48只,随机分为假手术组24只和球囊组24只。球囊损伤术后30天取材,血管组织作HE染色,光学显微镜观察病理学改变,免疫组织化学检测钙调神经磷酸酶在血管壁中的表达;ELISA法测定血清单核细胞趋化蛋白1(monocyte chemoattractant protein-1,MCP-1)水平。结果球囊损伤后血管壁出现新生内膜,形成的新生内膜厚度不均。球囊组与假手术组相比较内膜增生、内膜/中膜厚度明显增加(P<0.05)。免疫组织化学检测血管壁上钙调神经磷酸酶表达,球囊组与假手术组相比表达明显增加(P<0.05)。血浆炎症因子血清单核细胞趋化蛋白1水平球囊组与假手术组相比差异有统计学意义(P<0.05)。结论球囊扩张术导致大鼠腹主动脉内膜增生,同时伴随钙调神经磷酸酶蛋白表达明显增强以及血浆的炎症因子血清单核细胞趋化蛋白1水平升高,提示钙调神经磷酸酶信号通路和炎症因子血清单核细胞趋化蛋白1可能在球囊损伤后内膜增生过程中起重要作用。
Aim To investigate the alteration of expression of calcineurin in the abdominal aortas and levels of inflammatory cytokines injured by balloon in rats.Methods Fourty-eight healthy adult male SD rats were randomly divided into two groups： sham group（n=24） and balloon group（n=24）.Thirty days after injury,specimens were obtained from rats.HE staining and immunohistochemistry were performed to measure the expression level of CaN in arterial wall.Pathological changes of the arterial wall were observed by light microscope.The serum concentrations of MCP-1 was determined by Elisa.Results Intimal hyperplasia was observed in balloon injured rats,the neointima was not uniform in thickness.The thickness of the intimal layers,and the ratio of the intimal and the medial layers in the sham group was obviously lower than that in the balloon group（P0.05）.Immunohistochemistry assay showed that the expression of CaN was significantly increased in balloon group compared with that in the sham group（P0.05）.The serum concentrations of MCP-1 in balloon group is higher than that in sham group（P0.05）.Conclusions Intimal hyperplasia in rat abdominal aorta following balloon injury,accompanied by the expression of CaN protein and plasma inflammatory cytokines MCP-1 levels were significantly increased,which indicated that CaN signaling pathway and MCP-1 may play an important role in intimal hyperplasia caused by balloon injury.
出处
《中国动脉硬化杂志》
CAS
CSCD
北大核心
2012年第3期231-234,共4页
Chinese Journal of Arteriosclerosis
基金
广东省医学科学技术研究基金(A2006298)
广东省科技厅科技计划项目(2010B031600140)
