摘要
目的观察罗格列酮对大鼠颈动脉损伤后新生内膜及炎症因子的影响。方法将SD大鼠随机分为3组(即对照组、手术组、治疗组)。手术组及治疗组均用球囊导管扩张法损伤左侧颈总动脉,治疗组给予罗格列酮灌胃治疗。术后4h,1d和7d,用放免法检测大鼠血浆中肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6)水平。2周后取损伤血管行苏木精-伊红染色、免疫组化染色及光镜观察,计算内膜面积(NIA)、内弹力板围绕面积(IELA)、管腔狭窄指数(SI)及核转录因子-κB(NF-κB)蛋白表达的光密度值。结果罗格列酮能抑制损伤动脉新生内膜的形成,抑制NF-κB的蛋白表达,降低血浆中TNF-α和IL-6水平。结论罗格列酮可以通过抑制损伤血管的炎症反应,减轻损伤血管的再狭窄。
Objective To investigate the effect of rosiglitazone on neointima and inflammatory factor after carotid artery injury of rats. Methods A total of 60 SD rats were divided randomly into 3 groups ： the control group, the surgery group and the resiglitazone therapeutic group. The left common carotid arteries were injured by balloon in the surgery group and the therapeutic group. Rosiglitazone was administrated intragastricly to therapeutic group. The plasma tumor necrosis factor-α （TNF-α） and interleukin-6 （IL-6） levels were detected with radioimmunoassay at 4h, 1 and 7d after the operation. After 2 weeks, the injured vessels were stained by HE and immunity histochemistry method, then observed with microscope. Neointimal area （NIA）, internal elastic lamina area （IELA）, stenosis index （SI） and optical density value of protein expression of NF-KB were calculated. Results Rosiglitazone inhibited the neointima of injured vessels, decreased the protein expression of NF-KB and reduced plasma TNF-α and IL-6 levels. Conclusion Rosiglitazone can inhibit the inflammatory reaction of injured vessels and lighten the restenosis of injured vessels.
出处
《中国介入心脏病学杂志》
2007年第2期103-106,共4页
Chinese Journal of Interventional Cardiology
