摘要
目的:探讨单味中药柔毛淫羊藿(EPM)对去卵巢小鼠骨密度、破骨细胞和白介素-1β(IL-1β)的影响。方法:切除雌性小鼠双侧卵巢制备骨质疏松动物模型,随机分为模型组、己烯雌酚(DES,2 mg.kg-1)组及EPM水煎浸膏高、中、低剂量(200,100,50 mg.kg-1)组,另设正常对照组。术后45 d开始给予相应药物,1次/d,60 d后检测小鼠骨组织骨密度、破骨细胞数和骨组织中IL-1β。结果:模型组动物呈现明显的骨质疏松表现;给药组动物骨密度增加;EPM高、中、低剂量骨组织破骨细胞数分别为(1.9±1.0),(0.9±0.6),(0.8±0.4)个,明显低于模型组(3.4±1.6)个,(P<0.05或P<0.01);EPM高、中、低剂量骨组织IL-1β阳性细胞数分别为(11.6±2.1),(12.4±2.2),(16.4±2.7)个,明显低于模型组(20.6±3.2)个(P<0.01)。结论:EPM能增加去卵巢骨质疏松症小鼠的骨密度、降低破骨细胞数和IL-1β的表达。
Objective ： To investigate the effect of Epimedium pubescens Maxim. （EPM） on osteoporosis in ovariectomized mouse. Method： Kunming mice were used in this study. Osteoposis model was estabished by ectomizing the ovarians of the mice. The mice were divided into six groups randomly ： control group, model group, diethylstilbestrol positive control group, high dose of EPM group （200 mg. kg-1 ）, middle dose of EPM group （100 mg . kg-1） and low dose of EPM group （50 mg . kg-1）. Sixty days after administration, bone density, the osteoclast and intelukin （IL-1β） of the bone tissue were measured. Result： The model group animals had obvious osteoporosis. EMP increased bone density, and decreased the osteoclast number and IL-1β obviously. Conlusion： EPM can increase the bone density. EPM has an apparent role of anti-osteoporosis in ovariectomized mice. And it＇ s mechanism may be related to the reducing of the level of IL-1β.
出处
《中国实验方剂学杂志》
CAS
北大核心
2012年第6期241-243,共3页
Chinese Journal of Experimental Traditional Medical Formulae
