摘要
目的:通过测定切除卵巢骨质疏松模型小鼠骨组织中白介素6 (IL- 6)、白介素1β(IL- 1β)、肿瘤坏死因子α(TNF -α)相关细胞因子的表达特征,探讨其在骨质疏松发病过程中的生物学意义。方法:选用4月龄雌性BALB/C小鼠随机分假手术对照组和去卵巢骨质疏松模型组,术后第12周用ABC免疫组织化学方法测定骨组织中IL -6,IL- 1β,TNF -α的蛋白含量并进行细胞定位。结果:免疫组织化学染色结果显示, 3种蛋白在模型组和对照组均有阳性表达,主要位于骨小梁周边成骨细胞、破骨细胞及骨髓细胞胞浆中,模型组蛋白含量明显高于对照组且与骨密度呈负相关(P<0. 01或P<0. 05)。结论:小鼠骨质疏松模型骨组织相关破骨细胞分化因子IL- 6,IL -1β,TNF -α表达增高,导致破骨活动增强,骨吸收大于骨形成,最终导致骨质疏松。
Objective: To examine the expression level of interleukin-6(IL-6),interleukin-1β(IL-1β), and tumor necrosis factor-α(TNF-α) in mice models of experimental osteoporosis ,and to explore the molecular mechanism of osteoporosis.Methods:Four-month female BALB/C mice were randomly divided into ovariectomized group and sham-operated group.Twelve weeks later, levels of IL-6,IL-1β,and TNF-α in bone tissue were determined by using immunohistochemistry techniques.Results:The immunohistochemistry showed positive signals for protein of IL-6,IL-1β,and TNF-α in both groups. The distribution was in the cytoplasm of osteoblast,osteoclast, marrow stroma cell.The contents of protein in ovariectomized group were higher than those in sham-operated group,and the contents were negatively correlated with the bone mineral density(P< 0.01 or P<0.05).Conclusion:The expression levels and protein contents of IL-6,IL-1β,and TNF-α significantly increased in the bone tissue of ovariectomized mice.As a result, the differentiation of osteoclast increased and bone resorption is more stronger than bone formation.All of these may cause the occurrence of osteoporosis.
出处
《中国医科大学学报》
CAS
CSCD
北大核心
2005年第2期97-99,共3页
Journal of China Medical University
基金
国家自然科学基金资助项目(39900170)
关键词
骨质疏松
白介素6
白介素1Β
肿瘤坏死因子Α
骨密度
osteoporosis
interleukin-6
interleukin-1β
tumer necrosis factor-α
bone mineral density
