摘要
目的:研究金铁锁体外抗氧化活性。方法:采用清除采用清除二苯代苦味酰基(DPPH)自由基、清除[2,2'-连氨-(3-乙基苯并噻唑啉-6-磺酸)二氨盐](ABTS)自由基及铁离子还原/抗氧化能力(FRAP)测定法,对金铁锁体外抗氧化活性进行评价,并阳性对照没食子酸丙酯(PG)、丁基羟基茴香醚(BHA)和二丁基羟基甲苯(BHT)比较。结果:金铁锁3个提取物体外抗氧化活性均弱于阳性对照PG,BHA,BHA。在3个提取物中,金铁锁乙酸乙酯提取物清除ABTS自由基(IC50=40.54mg.L-1)及还原Fe3+的能力[TEAC=(696.9±2.42)μmol.g-1]较强;正丁醇清除ABTS自由基(IC50=83.38 mg.L-1)及还原Fe3+的能力[TEAC=(166.1±1.06)μmol.g-1]次之,石油醚提取物最弱。结论:金铁锁乙酸乙酯部位体外抗氧化活性较强。
Objective： To study the antioxidant activity Psammosilene tunicoides in vitro. Method： Antioxidant activities of P. tunicoides were evaluated by the 1, 1-diphenyl-2-picrylhydrazyl （DPPH） radical scavenging, [ 2, 2＇-azino-bis scavenging and ferric reducing （ 3-ethylbenzothiazoline ） antioxidant power （ FRA -6-sulphonic acid ] diamonium salt （ P） assay. Resuhs were compared wi ABTS ） that of radical positive controls PG, BHA and BHT. Result： Antioxidant activity of three extracts from P. tunicoides was weaker than that of the positive control PG, BHA and BHA. In the three extracts, Antioxidant activities of ethyl acetate extract was good. ABTS radical scavenging （ICs0 = 40. 54 mg , L- 1 ） and ferric reducing antioxidant power [ TEAC = （696. 9 ± 2.42）μmol·g-1 ] of ethyl acetate extract were strong, followed by the ABTS radical scavenging （ IC50 = 83.38 mg·L-1） and ferric reducing antioxidant power [TEAC = （166.1 -＋ 1.06） μmol·g-1] of n-butanol extract, petroleum ether extract was the weakest. Conclusion： Ethyl acetate extract of P. tunicoides had strong antioxidant activity.
出处
《中国实验方剂学杂志》
CAS
北大核心
2012年第6期109-112,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
教育部科学技术研究重点项目(210203)
