摘要
目的:建立一种从巴豆中提取纯化及制备巴豆苷的方法。方法:以巴豆苷得率为指标,采用正交试验设计优化巴豆苷的提取工艺参数;考察大孔树吸附脂纯化工艺条件;应用制备型液相色谱分离高纯度巴豆苷。结果:最佳巴豆苷提取工艺参数为8倍量20%乙醇提取3次,每次40 min;大孔树脂吸附初步纯化后,制备液相分离出巴豆苷单体,质量分数达98%,可作为对照品。结论:该方法简便易行,可用于分离制备高纯度巴豆苷样品。
Objective： To establish a method for extracting, purifying and preparing isoguanosine from Croton tiglium. Method： Orthogonal design was used to optimize extraction technology parameters with the content of isoguanosine as index; Studying purification process of isoguanosine with macroporous resin and preparing high purity isoguanosine by preparative liquid chromatography. Result： Optimum extraction technology of isoguanosine was extracted 3 times with 8 times the amount of 20% ethanol, 40 min each time. After preliminarily purifying by macroporous resin, isoguanosine was isolated from purified extracting solution and the purity was up to 98%. Conclusion： This method was simple and easy to operate, also could be used for preprartion high purity isoguanosine samples.
出处
《中国实验方剂学杂志》
CAS
北大核心
2012年第6期21-24,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家科技部重大新药创制专项(2009ZX09103-388)
关键词
巴豆
巴豆苷
提取
纯化
大孔树脂
制备液相
liquid chromatographyCroton tiglium
isoguanosine
extraction
purification
macroporous resin
preparative
