摘要
以甘蔗属优良甘蔗新品种(系)为母本,以收集于广西的河八王属材料为父本进行有性杂交,利用SSR标记对获得的杂种进行真实性鉴定。从70对甘蔗SSR引物中筛选出9对用于对5个甘蔗×河八王组合的57个杂交后代植株进行鉴定,结果表明,其中有14株具有双亲特征带,确定为真杂种。SSR标记可用于甘蔗河八王杂种真实性鉴定,研究结果为甘蔗野生种质河八王属植物的杂交利用及相关基础研究奠定了良好的基础。
In a bid to explore the feasibility of distant hybridization between sugarcane and Narenga porphyrocoma（Hance） Bor, SSR markers were used to identify the progenies from five crosses between sugarcane × Narenga porphyrocoma（Hance） Bor.Nine pairs of primers selected from 70 pairs of SSR primers were screened out and applied for identification of 57 progenies of sugarcane × Narenga porphyrocoma（Hance） Bor.The results showed that fourteen progenies had the special bands of parents,and they were thereby recognized as the true hybrids.SSR marker technology was proved to be useful for identifying the progenies of sugarcane × Narenga porphyrocoma（Hance） Bor.It could be concluded that this experiment established a favorable foundation on the effective use of hybrids from sugarcane and Narenga porphyrocoma（Hance） Bor as well as the related basic research.
出处
《西南农业学报》
CSCD
北大核心
2012年第1期38-43,共6页
Southwest China Journal of Agricultural Sciences
基金
国家自然科学基金项目(31101195
30971837
30860150)
国家科技支撑计划项目(2007BAD30B00)
科技部国际合作项目(2008DFA30600
2009DFA30820)
农业部948项目(2009-Z8)
现代农业产业技术体系项目(CARS-20-3)
广西自然科学基金项目(0991009Z
2010GXNSFB013027)
广西农科院科技发展基金项目(200906Z
200923
G2010005)
广西甘蔗研究所基本科研业务费项目(G2009008
G2009010
G2010005)
关键词
甘蔗
河八王
SSR
杂种鉴定
Sugercane
Narenga porphyrocoma（Hance） Bor
SSR marker
Hybrids identification
