摘要
目的探讨牛蒡子苷对晚期氧化蛋白产物(AOPP)诱导小鼠足细胞转分化的影响。方法用200μg/ml AOPP刺激小鼠足细胞24 h,同时加入牛蒡子苷(浓度分别为50、100、200、400μmol/L)进行干预,采用Western blotting检测内质网应激标志性蛋白Grp78、CHOP及平滑肌激动蛋白(α-SMA)表达水平。结果牛蒡子苷干预后可抑制小鼠足细胞Grp78、CHOP、α-SMA蛋白表达水平,且在一定范围内呈剂量依赖性。结论 AOPP可通过引起小鼠足细胞内质网应激(ERS)从而导致上皮-间充质转分化(EMT),而牛蒡子苷则可通过减轻ERS从而逆转EMT,为治疗肾脏纤维化提供新的研究方向。
Objective To study the effect of arctiin on mouse podocyte epithelial-mesenchymal transition（EMT） induced by advanced oxidation protein products（AOPP）.Methods Mouse podocytes were stimulated by 200 μg/ml AOPP for 24 h in the presence of 50,100,200,and 400 μmol/L arctiin.The expressions of α-smooth muscle actin,Grp78 and CHOP were detected using Western blotting.Result The expressions of α-SMA,Grp78 and CHOP were inhibited by arctiin,showing a dose-dependent effect within a given range of arctiin concentration.Conclusion AOPP causes endoplasmic reticulum stress to induce EMT of mouse podocytes,and arctiin can decrease EMT by alleviating the stress.This finding sheds light on a new scope of research of renal fibrosis.
出处
《南方医科大学学报》
CAS
CSCD
北大核心
2012年第3期379-382,共4页
Journal of Southern Medical University
基金
广东省自然科学基金(S2011010004053)
广州市科技计划重点项目(11C22120703)
关键词
牛蒡子苷
足细胞
上皮-间充质转分化
晚期氧化蛋白产物
arctiin
mouse podocytes
epithelial-mesenchymal transition
advanced oxidation protein products
