摘要
目的本实验以大型实验动物屏障设施为研究对象,分析屏障设施的环境微生物及尘埃粒子的动态变化规律及其相关影响因素。方法测定屏障设施各功能区域不同时间及不同工作状态下空气落下菌和直径≥0.3μm尘埃粒子的数量变化。结果屏障系统落下菌与尘埃粒子变化规律如下:屏障系统内空气落下菌与尘埃粒子在饲养工作后显著升高,喷雾消毒后明显降低;大、小鼠饲育室空气落下菌与尘埃数在凌晨时明显升高,而兔饲育室在凌晨时间段则较低;清洁走廊和污染走廊在工作状态时细菌含量明显上升,非工作状态时细菌含量一直处于较低的水平。结论实验动物屏障系统的环境微生物与尘埃粒子的动态数量变化与动物品种、空气消毒、人员进出及动物室内的饲养操作等有关。
Objective To study the dynamic changes of becteria and the dust particles in barrier animal facilities.Methods To measure the quantity of falling bacteria and dust particles of larger than or equal 0.3 μm in diameter at different times and in the different functional areas of the barrier animal facilities.Results The quantity of falling bacteria and dust particles were significantly increased in barrier animal room after breeding operation,and was the lowest after spray disinfection.There was a high quantity of bacteria and dust at 00∶00 in the mice and rats breeding room,but was a valley value in the rabbits breeding room at the same time.The quantity of falling bacteria and dust particles was significantly increased in the clean and semi-clean corridor at working time,and was kept a relatively stable level at non-working times.Conclusions There are regular changes of the quantity of falling bacteria and dust particles in barrier animal facilities,and these changes are related with animal species,spray disinfection,mobility of persons and materials and operation of animal care.
出处
《中国比较医学杂志》
CAS
2012年第2期61-65,共5页
Chinese Journal of Comparative Medicine
基金
国家科技支撑项目2006BAI23B04
上海市科研计划项目08140900800
关键词
落下菌
屏障系统
尘埃粒子
实验动物
Falling bacteria
Barrier animal facilities
Dust
Laboratory animal
