摘要
从吉林省延边地区的家兔的脏器组织分离出了13株流行性出血热病毒。其中对乳鼠脑腔接种分离出的3株病毒进行了系统的鉴定。这些病毒株的抗原与流行性出血热病人的双份血清和病兔的恢复期血清进行试验时,血清的抗体滴度有4倍或4倍以上增长,而不能对正常人和呼肠弧病毒Ⅰ～Ⅲ型血清发生反应。毒株与流行性出血热病毒单克隆抗体进行间接免疫荧光试验时证明,从家兔分离出的JR_1、JR_2、和JR_3株病毒是姬鼠型流行性出血热病毒。
13 strains of EHF virus were isolated from organs of 4 infected rabbits entrapped from the EHF endemic area in YanBian of Jilin province. Among them 3 strains isolated by intracerebrally into suckling mice were systematically identified. These strains were used as antigen to test the paired sera from the EHF patients and convalescent serum of the infected rabbits. The test showed 4 fold or more rise in antibody titer, but the strains used as antigen did not react with sera of normal person's and sera of reoviruses tyqes 1—3. Antigenic analysis of these strains were carried out using indirect immuno-fluorescent antibody technique by EHF McAbs, The results showed that JR1, JR2 and JR3 strains isolated from rabbits belonged to EHF Virus Apodemirs tyqe.
