摘要
目的测定鸡粪中Ｔ－２四醇、Ｔ－２三醇、Ｔ－２毒素、ＨＴ－２毒素的含量。方法人工染毒，化学提取，ＴＢＴ衍生，宽田径毛细管柱ＧＣ测定，投予剂量不同回收率略有差异。结果该方法的回收率 ２００ｎｇＴ－２四醇８３．１％～９２． ８％；Ｔ－２三醇 ８３． ６％～９０． ６％；Ｔ－２毒素、ＨＴ－２毒素 ７９． ６％～９０． ６％。结论方法的回收率高，操作简单，可供研究Ｔ－２毒素代谢机制应用。
Objective Detect the content of T-2tetraol, T-2 triol, T-2 txoin and HT-2 toxin in chicken excreta. Methods Chickens are feed with T-2 toxin by intraperitoneal injection. The metabolites are extracted by acetonitrile and derivated by TBT,then detected with the wide caliber GC.Results The average recoveries are 83.1% -92.8% for T-2 tetraol; 83.6%-90. 6% for T-2 triol and 79.6%-90.6% for T-2 toxin and HT-2 toxin.Conclusions The method is simple.The average recoveries are high. The method provides a good tool for detection of T-2 toxin metablolites in bio-excreta.
出处
《中国地方病学杂志》
CAS
CSCD
2000年第2期142-144,共3页
Chinese Jouranl of Endemiology
