摘要
目的:建立HPLC法同时测定血清中利福平和利福喷丁的血药浓度。方法:以对硝基酚为内标,采用Shim-pack VP-ODS(150 mm×4.6 mm)为分析柱,以甲醇-水(70∶30)为流动相,流速为1 ml/min,检测波长为336 nm。结果:利福平和利福喷丁的线性范围分别为0.25～32.0 mg/L(r=0.999 5)和0.25～32.0 mg/L(r=0.999 6),平均回收率分别为100.4%和99.8%。日内及日间RSD均小于5%。结论:方法、简便准确,适用于对利福平和利福喷丁同时进行治疗药物监测的需要,测定时可以有效地降低成本、提高效率。
Objective：To establish a HPLC method for stimulanteous determination the concentration of rifampin and rifapentine in human plasma. Method： Using d - nitrophenol as the internal standard. HPLC condition： analytical column： Shim - pack VP - ODS（ 150 mm ×4.6 mm ）, the mobile phase：methanol -water （70： 30） ,the flow rate：1 ml/min,the wavelength of UV detec- tion： 336 nm. Results ： The linear ranges of rifampin and rifapentine were at of 0.25 - 32.0 mg/L （ r = 0. 999 5 ） 和 0.25 - 32.0 mg/L （r =0.999 6） respectively. The average recoveries of rifampin and rifapentine were 100.4% and 99.8% respectively. The PtSD of within - day and between - days were all less than 5%. Conclusion：This method is quite sensitive,simple and accurate. It is suitable to be used in determining the concentration of rifampin and rifapentine in human plasma. The costs can be reduced and the efficiency can be raised.
出处
《天津药学》
2012年第1期20-21,共2页
Tianjin Pharmacy
