摘要
目的研究CSF1PO、TH01和TPOX复合扩增最佳体系。方法设计正交实验进行聚合酶链式反应(PCR),应用聚丙烯酰胺凝胶电泳分离显带技术检测扩增片段。结果 最佳反应体系组成为:10 × PCRbuffer2.5μl、0.72 mmol/L dNTP、2.5 mmoL/L MgCl2、2.0 U Taq酶,各基因座引物终浓度(μmol/L)CSF1PO:0.4,TH01:0.4,TPOX:0.6。结论获得复合扩增条件各反应因素较为满意的参数。
Objective To investigate the mutiplex amplification condition of STR loci CSF1PO, TH01 and TPOX. Methods PCR were performed according to the data of contingency test. The products were checked by using PAGE and silver stains. Results The experiment suggested that the best reaction system was : 10 × PCR buffer 2. 5 μl, 0. 72 mmol/L dNTP, 2. 5 mmol/L MgCl2, 2. 0 U Taq polymerrase, and the concentrations of CSF1PO, TH01, TPOX were 0.4 μmol/L、 0. 4μmol/L、 0.6 μmol/L, respectively. Conclusion The satisfactory parameters for the mutiplex amplification were obtained in the study.
出处
《医学分子生物学杂志》
CAS
CSCD
2004年第1期33-35,共3页
Journal of Medical Molecular Biology
