摘要
目的探讨流式细胞术检测血小板相关抗体在特发性血小板减少性紫癜诊治中的价值。方法用流式细胞术、ELISA法测定45例ITP患者及20例体检正常人的血小板相关抗体(PAIgG、PAIgM、PAIgA)的水平,并分别进行ITP患者与正常人进行配对比较。结果 FCM检测20例正常人血小板表面IgG、IgA和IgM的荧光百分率分别为(15.21±8.83)%,(4.89±7.48)%,(13.51±6.21)%;ITP患者血小板表面IgG、IgA和IgM荧光百分率分别为(53.21±18.92)%,(45.95±20.21)%,(22.18±15.77)%。结论血小板特异抗体检测对肯定ITP诊断有重要的意义,流式细胞术可快速、敏感、准确地测定PAIg,且明显优于ELISA法。
Objective To investigate the value of flow cytometry （FCM）. method in the diagnosis of idiopathic thrombocytopenie purpura（ITP）. Methods FCM and ELISA method were used for detection of platelet associated antibody in 45 patients with ITP and 20 healthy controls. The results of FCM were compared with that of ELISA. Results By FCM,the levels of platelet associated IgG,IgM and IgA in 20 normal controls were 15.21 ± 8.83% ,4.89 ± 7.48%, 13.51 ± 6.21%. while the levels of platelet associated IgG,IgM and IgA in ITP patients were 53.21 ± 18.92% ,45.95 ±20.21% and 22.18 ± 15.77%. Conclusion The detection of specific platelet associated antibody is of great significance in confirmation of ITP and FCM is a rapid, sensitive method in detecting platelet associated antibody and supior to ELISA.
出处
《中国热带医学》
CAS
2012年第1期82-83,共2页
China Tropical Medicine
