摘要
目的研究慢性脑缺血大鼠海马组织中细胞分裂周期蛋白42(Cdc42)表达的变化,探讨其在慢性脑缺血所致认知功能障碍中可能发挥的作用。方法大鼠40只,随机分为假手术组、持久性双侧颈总动脉结扎(2VO)8、10、12 w组,每组各10只。应用Morris水迷宫检测大鼠学习记忆能力,用免疫组织化学及Western印迹两种方法检测各组大鼠海马区Cdc42的表达。结果 Morris水迷宫显示手术组大鼠较假手术组大鼠逃避潜伏期明显延长(P<0.05);免疫组化及Western印迹均显示手术组大鼠海马区Cdc42表达明显低于假手术组(P<0.05)。结论 Cdc42表达量的降低可能参与了慢性脑缺血所致认知功能障碍的形成。
Objective To investigate the expression of cell division cycle 42 GTP-binding protein （Cdc42） in hippocampus of chro- nic cerebral hypoperfusion rats model and explore the relationship between Cdc42 protein and cognitive impairment. Methods 40 rats were equally divided into sham and 2-VO model groups （permanent ligation of bilateral common carotid arteries for 8, 10 and 12 weeks, respec- tively）. The brains were taken at week 8,10 and 12 after the establishment of models for measuring the expression of Cde42 protein using Western blotting and immunohistochemistry, cognitive function including learning and memory capacity in rats was evaluated by Morris water maze at the same time after cerebral ischemia. Results In the Morris water maze, the latency of model rats obviously extended compared to that of control rats （P 〈 0. 05）, immunohistochemistry and Western blot all found that the expression of Cdc42 on hippocampal areas in the model rats was significantly decreased than that in control rats （P 〈 0. 05）. Conclusions Low expression of Cdc42 in the hippocampus may play an important role in cognitive dysfunction induced by chronic cerebral ischemia.
出处
《中国老年学杂志》
CAS
CSCD
北大核心
2012年第5期954-956,共3页
Chinese Journal of Gerontology
基金
国家自然科学基金资助项目(No.81000530)
河南省卫生厅项目(No.200903028)
关键词
CDC42
慢性脑缺血
海马
认知功能
Chronic cerebral ischemia
Hippocampus
Cdc42
Cognitive function
