摘要
为研究鸦胆子油乳对子宫内膜异位症模型大鼠血管内皮生长因子(Cyelooxygenase-2,COX-2)表达的影响及其机理,以雌性Wistar大鼠为研究对象,用自体移植法制作子宫内膜异位症模型,用鸦胆子油乳腹腔给药治疗,疗程结束后观察异位囊肿消失情况,并用免疫组化及蛋白印迹法检测子宫组织中COX-2表达及含量变化情况。结果显示:鸦胆子油乳能使子宫内膜异位囊肿明显减小甚至消失,能抑制子宫内膜组织中COX-2表达。由此得出结论:鸦胆子油乳对子宫内膜异位症大鼠异位囊肿有治疗作用,可能是通过降低COX-2表达,抑制血管生成,促进细胞凋亡起作用。
To explore the effect mechanism of bruceolic oil emulsion （BOE） on expressions of cyclooxygenase - 2 （ COX - 2） in rats with endometriosis . The Wistar rat model of endometriosis was established by surgical implant of endometrial tissue which belonged to its body. The changes of endometriotic cyst were observed after treatment by intraperitoneal injection. And then COX -2 expression in endometriotic tissue was measured by immunohistochemistry and western blotting respectively. The results showed that endometriotic cyst was significantly reduced or disappeared by BOE and there was a decrease in the expression of COX - 2. In conclusion, the mechanism of BOE for endometriosis in rats may be related to the decrease of COX -2 expression in endometrium, inhibition of angiogenesis, and oromoting anootosis.
出处
《中华中医药学刊》
CAS
2012年第2期355-357,I0014,共4页
Chinese Archives of Traditional Chinese Medicine
基金
黑龙江省教育厅科学研究资助项目(11511356)
关键词
鸦胆子油乳
子宫内膜异位症
环氧合酶-2
免疫组化
蛋白印迹
bruceolic oil emulsion
endometriosis
cyelooxygenase - 2
immunohisto - chemistry
Western blotting
